CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004488
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 General transcriptional corepressor TUP1 
Protein Synonyms/Alias
 Flocculation suppressor protein; Glucose repression regulatory protein TUP1; Repressor AER2 
Gene Name
 TUP1 
Gene Synonyms/Alias
 AAR1; AER2; AMM1; CYC9; FLK1; SFL2; UMR7; YCR084C; YCR84C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
39TYRLQNQKDYDFKMNacetylation[1]
229TTTLPSVKAPESTLKacetylation[1]
236KAPESTLKETEPENNacetylation[1]
236KAPESTLKETEPENNubiquitination[2]
247PENNNTSKINDTGSAacetylation[1]
270TETEIKPKEEDATPAsumoylation[3]
365YLATGCNKTTQVYRVubiquitination[2]
601GSLDRSVKLWNLQNAubiquitination[2]
659RGVLFWDKKSGNPLLubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Identification of sumoylated proteins by systematic immunoprecipitation of the budding yeast proteome.
 Wykoff DD, O'Shea EK.
 Mol Cell Proteomics. 2005 Jan;4(1):73-83. [PMID: 15596868] 
Functional Description
 Acts as component of the CYC8-TUP1 corepressor complex which is involved in the repression of many genes in a wide variety of physiological processes including heme-regulated and catabolite repressed genes. May also be involved in the derepression of at least some target genes. The complex is recruited to target genes by interaction with DNA-bound transcriptional repressors, like MATALPHA2, MIG1, RFX1 and SKO1. The complex recruits histone deacetylases to produce a repressive chromatin structure, interacts with hypoacetylated N-terminal tails of histones H3 and H4 that have been programmed for repression by the action of histone deacetylases and interferes directly with the transcriptional machinery by associating with the RNA polymerase II mediator complex. 
Sequence Annotation
 REPEAT 342 371 WD 1.
 REPEAT 441 471 WD 2.
 REPEAT 483 513 WD 3.
 REPEAT 524 555 WD 4.
 REPEAT 574 604 WD 5.
 REPEAT 628 658 WD 6.
 REPEAT 670 706 WD 7.
 MOD_RES 439 439 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 713 AA 
Protein Sequence
MTASVSNTQN KLNELLDAIR QEFLQVSQEA NTYRLQNQKD YDFKMNQQLA EMQQIRNTVY 60
ELELTHRKMK DAYEEEIKHL KLGLEQRDHQ IASLTVQQQR QQQQQQQVQQ HLQQQQQQLA 120
AASASVPVAQ QPPATTSATA TPAANTTTGS PSAFPVQASR PNLVGSQLPT TTLPVVSSNA 180
QQQLPQQQLQ QQQLQQQQPP PQVSVAPLSN TAINGSPTSK ETTTLPSVKA PESTLKETEP 240
ENNNTSKIND TGSATTATTT TATETEIKPK EEDATPASLH QDHYLVPYNQ RANHSKPIPP 300
FLLDLDSQSV PDALKKQTND YYILYNPALP REIDVELHKS LDHTSVVCCV KFSNDGEYLA 360
TGCNKTTQVY RVSDGSLVAR LSDDSAANNH RNSITENNTT TSTDNNTMTT TTTTTITTTA 420
MTSAAELAKD VENLNTSSSP SSDLYIRSVC FSPDGKFLAT GAEDRLIRIW DIENRKIVMI 480
LQGHEQDIYS LDYFPSGDKL VSGSGDRTVR IWDLRTGQCS LTLSIEDGVT TVAVSPGDGK 540
YIAAGSLDRA VRVWDSETGF LVERLDSENE SGTGHKDSVY SVVFTRDGQS VVSGSLDRSV 600
KLWNLQNANN KSDSKTPNSG TCEVTYIGHK DFVLSVATTQ NDEYILSGSK DRGVLFWDKK 660
SGNPLLMLQG HRNSVISVAV ANGSPLGPEY NVFATGSGDC KARIWKYKKI APN 713 
Gene Ontology
 GO:0017053; C:transcriptional repressor complex; IDA:SGD.
 GO:0042393; F:histone binding; IDA:SGD.
 GO:0042826; F:histone deacetylase binding; IDA:SGD.
 GO:0036033; F:mediator complex binding; IDA:SGD.
 GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; IDA:SGD.
 GO:0001191; F:RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription; IDA:SGD.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0043486; P:histone exchange; IMP:SGD.
 GO:0035955; P:negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0001198; P:negative regulation of mating-type specific transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0000433; P:negative regulation of transcription from RNA polymerase II promoter by glucose; IMP:SGD.
 GO:0007070; P:negative regulation of transcription from RNA polymerase II promoter during mitosis; IMP:SGD.
 GO:0016584; P:nucleosome positioning; IDA:SGD.
 GO:2000531; P:regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:2001020; P:regulation of response to DNA damage stimulus; IMP:SGD.
 GO:0061418; P:regulation of transcription from RNA polymerase II promoter in response to hypoxia; IMP:SGD.
 GO:0061392; P:regulation of transcription from RNA polymerase II promoter in response to osmotic stress; IMP:SGD. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR013890; Tscrpt_rep_Tup1_N.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF08581; Tup_N
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.