CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018593
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DnaJ homolog subfamily C member 3 
Protein Synonyms/Alias
 Interferon-induced, double-stranded RNA-activated protein kinase inhibitor; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58 
Gene Name
 Dnajc3 
Gene Synonyms/Alias
 P58ipk 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
96AALPDLTKVIALKMDubiquitination[1]
200LRAECFIKEGEPRKAacetylation[2]
270KQVKKLNKLIESAEEubiquitination[1]
290RYTDATSKYESVMKTubiquitination[1]
296SKYESVMKTEPSVAEubiquitination[1]
318RICHCFSKDEKPVEAacetylation[2]
344PDNVNALKDRAEAYLubiquitination[1]
446FIDIAAAKEVLSDPEubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. 
Sequence Annotation
 REPEAT 37 70 TPR 1.
 REPEAT 72 104 TPR 2.
 REPEAT 105 138 TPR 3.
 REPEAT 154 187 TPR 4.
 REPEAT 188 221 TPR 5.
 REPEAT 222 255 TPR 6.
 REPEAT 268 301 TPR 7.
 REPEAT 306 339 TPR 8.
 REPEAT 340 373 TPR 9.
 DOMAIN 394 462 J.
 REGION 375 393 Flexible linker (By similarity).
 DISULFID 248 258 By similarity.
 DISULFID 313 329 By similarity.  
Keyword
 3D-structure; Chaperone; Complete proteome; Disulfide bond; Endoplasmic reticulum; Reference proteome; Repeat; Signal; TPR repeat; Unfolded protein response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 504 AA 
Protein Sequence
MVAPGSVGSR LGAVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA GQLADALSQF 60
HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIAL KMDFTAARLQ RGHLLLKQGK 120
LDEAEDDFKK VLKSNPSEQE EKEAESQLVK ADEMQRLRSQ ALDAFDGADY TAAITFLDKI 180
LEVCVWDAEL RELRAECFIK EGEPRKAISD LKAASKLKSD NTEAFYKIST LYYQLGDHEL 240
SLSEVRECLK LDQDHKRCFA HYKQVKKLNK LIESAEELIR DGRYTDATSK YESVMKTEPS 300
VAEYTVRSKE RICHCFSKDE KPVEAIRICS EVLQMEPDNV NALKDRAEAY LIEEMYDEAI 360
QDYEAAQEHN ENDQQIREGL EKAQRLLKQS QKRDYYKILG VKRNAKKQEI IKAYRKLALQ 420
WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR 480
SWNSWQGFNP FSSGGPFRFK FHFN 504 
Gene Ontology
 GO:0005829; C:cytosol; TAS:BHF-UCL.
 GO:0005788; C:endoplasmic reticulum lumen; IDA:BHF-UCL.
 GO:0051787; F:misfolded protein binding; IDA:BHF-UCL.
 GO:0004860; F:protein kinase inhibitor activity; ISS:MGI.
 GO:0030968; P:endoplasmic reticulum unfolded protein response; IC:BHF-UCL.
 GO:0051603; P:proteolysis involved in cellular protein catabolic process; IDA:BHF-UCL. 
Interpro
 IPR001623; DnaJ_domain.
 IPR026901; DNAJC3.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00226; DnaJ
 PF00515; TPR_1 
SMART
 SM00271; DnaJ
 SM00028; TPR 
PROSITE
 PS00636; DNAJ_1
 PS50076; DNAJ_2
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS
 PR00625; JDOMAIN.