CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004785
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cytochrome c oxidase subunit 5B, mitochondrial 
Protein Synonyms/Alias
 Cytochrome c oxidase polypeptide Vb 
Gene Name
 Cox5b 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
56EIMIAAQKGLDPYNMacetylation[1, 2, 3]
56EIMIAAQKGLDPYNMubiquitination[4]
67PYNMLPPKAASGTKEacetylation[1, 3, 5, 6]
73PKAASGTKEDPNLVPacetylation[1, 3, 5, 6]
73PKAASGTKEDPNLVPubiquitination[4]
85LVPSISNKRIVGCICacetylation[1, 3, 6, 7]
85LVPSISNKRIVGCICsuccinylation[7]
85LVPSISNKRIVGCICubiquitination[4]
106VIWFWLHKGESQRCPacetylation[5, 6]
120PNCGTHYKLVPHQMAacetylation[3, 6]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [7] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
 This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. 
Sequence Annotation
 METAL 90 90 Zinc (By similarity).
 METAL 92 92 Zinc (By similarity).
 METAL 112 112 Zinc (By similarity).
 METAL 115 115 Zinc (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; Membrane; Metal-binding; Mitochondrion; Mitochondrion inner membrane; Reference proteome; Transit peptide; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 128 AA 
Protein Sequence
MASRLLRGVG ALAAQALRRT ARGAAVTRSM ASGGGVPTDE EQATGLEREI MIAAQKGLDP 60
YNMLPPKAAS GTKEDPNLVP SISNKRIVGC ICEEDNCTVI WFWLHKGESQ RCPNCGTHYK 120
LVPHQMAH 128 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. 
Interpro
 IPR002124; Cyt_c_oxidase_su5b.
 IPR020893; Cyt_c_oxidase_su5b_Zn_BS. 
Pfam
 PF01215; COX5B 
SMART
  
PROSITE
 PS00848; COX5B_1
 PS51359; COX5B_2 
PRINTS