UniProt Accession

 XAF1_HUMAN; Q6GPH4; A2T931; A2T932; A8K2L1; A8K9Y3; D3DTM6; Q6MZE8; Q8N557; Q99982 

Genbank Protein ID

 X99699; EF028165; EF028166; AK290276; AK292710; AK292848; CH471108; CH471108; BC032776; BC073156; BX649188 

Genbank Nucleotide ID

 CAA68030.1; ABM74556.1; ABM74557.1; BAF82965.1; BAF85399.1; BAF85537.1; EAW90288.1; EAW90289.1; AAH32776.1; AAH73156.1; CAE46206.1 

Protein Name

 XIAP-associated factor 1 

Protein Synonyms/Alias

 BIRC4-binding protein 

Gene Name

 XAF1 

Gene Synonyms/Alias

 BIRC4BP; XIAPAF1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
75	KSSLEFHKANECQER	ubiquitination	[1]
140	SEQAQLGKGERISAP	ubiquitination	[1]
171	KYFHHMGKCCPDSEF	ubiquitination	[1]
180	CPDSEFKKHFPVGNP	ubiquitination	[1]
237	KAPRSKNKTLDPLLM	ubiquitination	[1]
258	TSSPRGDKAAYDILR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. 

Sequence Annotation

 ZN_FING 22 99 TRAF-type.  

Keyword

 Alternative splicing; Apoptosis; Complete proteome; Cytoplasm; Metal-binding; Mitochondrion; Nucleus; Polymorphism; Reference proteome; Tumor suppressor; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 301 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0035456; P:response to interferon-beta; IDA:BHF-UCL.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome. 

Interpro

 IPR001293; Znf_TRAF. 

Pfam

  

SMART

  

PROSITE

 PS50145; ZF_TRAF 

PRINTS