UniProt Accession

 SIP2_YEAST; P34164; D6VTU7 

Genbank Protein ID

 Z14128; L31592; Z72730; U33754; BK006941 

Genbank Nucleotide ID

 CAA78503.1; AAC37420.1; CAA96922.1; AAC49497.1; DAA07908.1 

Protein Name

 SNF1 protein kinase subunit beta-2 

Protein Synonyms/Alias

 Protein SPM2; SNF1-interacting protein 2 

Gene Name

 SIP2 

Gene Synonyms/Alias

 SPM2; YGL208W; G1155 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
12	TSHPAQKKQTTKKCR	acetylation	[1]
16	AQKKQTTKKCRAPIM	acetylation	[1]
17	QKKQTTKKCRAPIMS	acetylation	[1]
29	IMSDVREKPSNAQGC	ubiquitination	[2]
55	VTELSLNKCSDSQDA	ubiquitination	[2]
256	EKNPTNEKIRSKEAD	acetylation	[1]

Reference

 [1] Acetylation of yeast AMPK controls intrinsic aging independently of caloric restriction.
 Lu JY, Lin YY, Sheu JC, Wu JT, Lee FJ, Chen Y, Lin MI, Chiang FT, Tai TY, Berger SL, Zhao Y, Tsai KS, Zhu H, Chuang LM, Boeke JD.
 Cell. 2011 Sep 16;146(6):969-79. [PMID: 21906795]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Beta subunit of the SNF1 kinase complex, which is required for transcriptional, metabolic, and developmental adaptations in response to glucose limitation. Has a structural role, mediating heterotrimer formation, and a regulatory role, defining carbon source-regulated subcellular location and substrate specificity of the SNF1 kinase complex. Involved in the regulation of aging. Acts as a negative regulator of nuclear SNF1 activity in young cells by sequestering its activating gamma subunit at the plasma membrane. 

Sequence Annotation

 REGION 154 335 Kinase-interacting sequence (KIS);
 REGION 336 415 Association with SNF1 kinase complex
 MOD_RES 66 66 Phosphoserine.
 MOD_RES 298 298 Phosphoserine.
 LIPID 2 2 N-myristoyl glycine.  

Keyword

 3D-structure; Cell membrane; Complete proteome; Cytoplasm; Lipoprotein; Membrane; Myristate; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 415 AA 

Protein Sequence

Gene Ontology

 GO:0031588; C:AMP-activated protein kinase complex; IDA:SGD.
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0042149; P:cellular response to glucose starvation; IMP:SGD.
 GO:0001403; P:invasive growth in response to glucose limitation; IGI:SGD.
 GO:0006468; P:protein phosphorylation; IGI:SGD.
 GO:0043254; P:regulation of protein complex assembly; IGI:SGD.
 GO:0001302; P:replicative cell aging; IMP:SGD.
 GO:0007165; P:signal transduction; IGI:SGD. 

Interpro

 IPR006828; AMP_prot_kin_bsu_interact-dom. 

Pfam

 PF04739; AMPKBI 

SMART

 SM01010; AMPKBI 

PROSITE

  

PRINTS