CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019826
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Endophilin-A2 
Protein Synonyms/Alias
 EEN fusion partner of MLL; Endophilin-2; Extra eleven-nineteen leukemia fusion gene protein; EEN; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1 
Gene Name
 SH3GL1 
Gene Synonyms/Alias
 CNSA1; SH3D2B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
7*MSVAGLKKQFYKASubiquitination[1]
8MSVAGLKKQFYKASQubiquitination[1]
102ECMIRHGKELGGESNubiquitination[2]
177YKKKRQGKIPDEELRubiquitination[1]
239ILDELAEKLKRRMREubiquitination[2, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Implicated in endocytosis. May recruit other proteins to membranes with high curvature (By similarity). 
Sequence Annotation
 DOMAIN 18 249 BAR.
 DOMAIN 306 365 SH3.
 REGION 1 21 Membrane-binding amphipathic helix (By
 REGION 60 87 Required for dimerization upon membrane
 REGION 218 254 Interaction with ARC (By similarity).
 MOD_RES 288 288 Phosphoserine.  
Keyword
 Alternative splicing; Cell junction; Cell projection; Chromosomal rearrangement; Coiled coil; Complete proteome; Cytoplasm; Endocytosis; Endosome; Lipid-binding; Membrane; Phosphoprotein; Proto-oncogene; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 368 AA 
Protein Sequence
MSVAGLKKQF YKASQLVSEK VGGAEGTKLD DDFKEMEKKV DVTSKAVTEV LARTIEYLQP 60
NPASRAKLTM LNTVSKIRGQ VKNPGYPQSE GLLGECMIRH GKELGGESNF GDALLDAGES 120
MKRLAEVKDS LDIEVKQNFI DPLQNLCEKD LKEIQHHLKK LEGRRLDFDY KKKRQGKIPD 180
EELRQALEKF EESKEVAETS MHNLLETDIE QVSQLSALVD AQLDYHRQAV QILDELAEKL 240
KRRMREASSR PKREYKPKPR EPFDLGEPEQ SNGGFPCTTA PKIAASSSFR SSDKPIRTPS 300
RSMPPLDQPS CKALYDFEPE NDGELGFHEG DVITLTNQID ENWYEGMLDG QSGFFPLSYV 360
EVLVPLPQ 368 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0042995; C:cell projection; IEA:UniProtKB-KW.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0002102; C:podosome; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0007417; P:central nervous system development; TAS:ProtInc.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR027267; AH/BAR-dom.
 IPR004148; BAR_dom.
 IPR000108; p67phox.
 IPR001452; SH3_domain. 
Pfam
 PF03114; BAR
 PF00018; SH3_1 
SMART
 SM00721; BAR
 SM00326; SH3 
PROSITE
 PS51021; BAR
 PS50002; SH3 
PRINTS
 PR00499; P67PHOX.
 PR00452; SH3DOMAIN.