CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017613
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sn1-specific diacylglycerol lipase beta 
Protein Synonyms/Alias
 DGL-beta; KCCR13L 
Gene Name
 DAGLB 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
509VTNLEDLKRRILRVVubiquitination[1]
628SHEAEFSKILIGPKMubiquitination[2, 3]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Catalyzes the hydrolysis of diacylglycerol (DAG) to 2- arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses. 
Sequence Annotation
 ACT_SITE 443 443 Charge relay system.
 ACT_SITE 495 495 Charge relay system.
 MOD_RES 570 570 Phosphoserine.
 MOD_RES 574 574 Phosphoserine.
 MOD_RES 579 579 Phosphoserine.
 MOD_RES 584 584 Phosphoserine.  
Keyword
 Alternative splicing; Calcium; Cell membrane; Complete proteome; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 672 AA 
Protein Sequence
MPGMVLFGRR WAIASDDLVF PGFFELVVRV LWWIGILTLY LMHRGKLDCA GGALLSSYLI 60
VLMILLAVVI CTVSAIMCVS MRGTICNPGP RKSMSKLLYI RLALFFPEMV WASLGAAWVA 120
DGVQCDRTVV NGIIATVVVS WIIIAATVVS IIIVFDPLGG KMAPYSSAGP SHLDSHDSSQ 180
LLNGLKTAAT SVWETRIKLL CCCIGKDDHT RVAFSSTAEL FSTYFSDTDL VPSDIAAGLA 240
LLHQQQDNIR NNQEPAQVVC HAPGSSQEAD LDAELENCHH YMQFAAAAYG WPLYIYRNPL 300
TGLCRIGGDC CRSRTTDYDL VGGDQLNCHF GSILHTTGLQ YRDFIHVSFH DKVYELPFLV 360
ALDHRKESVV VAVRGTMSLQ DVLTDLSAES EVLDVECEVQ DRLAHKGISQ AARYVYQRLI 420
NDGILSQAFS IAPEYRLVIV GHSLGGGAAA LLATMLRAAY PQVRCYAFSP PRGLWSKALQ 480
EYSQSFIVSL VLGKDVIPRL SVTNLEDLKR RILRVVAHCN KPKYKILLHG LWYELFGGNP 540
NNLPTELDGG DQEVLTQPLL GEQSLLTRWS PAYSFSSDSP LDSSPKYPPL YPPGRIIHLQ 600
EEGASGRFGC CSAAHYSAKW SHEAEFSKIL IGPKMLTDHM PDILMRALDS VVSDRAACVS 660
CPAQGVSSVD VA 672 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004806; F:triglyceride lipase activity; IEA:InterPro.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0030168; P:platelet activation; TAS:Reactome. 
Interpro
 IPR002921; Lipase_3. 
Pfam
 PF01764; Lipase_3 
SMART
  
PROSITE
 PS00120; LIPASE_SER 
PRINTS