UniProt Accession

 MDHC_MOUSE; P14152; Q3TP22; Q80Y13; Q9DB45 

Genbank Protein ID

 M29462; M36084; AK005237; AK164785; AK168545; AL663049; BC050940 

Genbank Nucleotide ID

 AAA39510.1; AAA37423.1; BAB23897.1; BAE37915.1; BAE40421.1; CAI24411.1; AAH50940.2 

Protein Name

 Malate dehydrogenase, cytoplasmic 

Protein Synonyms/Alias

 Cytosolic malate dehydrogenase 

Gene Name

 Mdh1 

Gene Synonyms/Alias

 Mor2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
103	MERKDLLKANVKIFK	acetylation	[1]
103	MERKDLLKANVKIFK	ubiquitination	[2]
107	DLLKANVKIFKSQGT	acetylation	[1]
110	KANVKIFKSQGTALE	ubiquitination	[2]
118	SQGTALEKYAKKSVK	acetylation	[1]
118	SQGTALEKYAKKSVK	ubiquitination	[2]
125	KYAKKSVKVIVVGNP	ubiquitination	[2]
142	TNCLTASKSAPSIPK	ubiquitination	[2]
149	KSAPSIPKENFSCLT	acetylation	[3]
149	KSAPSIPKENFSCLT	ubiquitination	[2]
164	RLDHNRAKSQIALKL	acetylation	[3]
170	AKSQIALKLGVTADD	ubiquitination	[2]
179	GVTADDVKNVIIWGN	ubiquitination	[2]
199	YPDVNHAKVKLQGKE	acetylation	[3]
199	YPDVNHAKVKLQGKE	ubiquitination	[2]
205	AKVKLQGKEVGVYEA	acetylation	[3, 4]
205	AKVKLQGKEVGVYEA	ubiquitination	[2]
214	VGVYEALKDDSWLKG	acetylation	[1, 3, 4, 5]
214	VGVYEALKDDSWLKG	succinylation	[5]
214	VGVYEALKDDSWLKG	ubiquitination	[2]
220	LKDDSWLKGEFITTV	acetylation	[3, 6]
220	LKDDSWLKGEFITTV	ubiquitination	[2]
239	AAVIKARKLSSAMSA	acetylation	[3]
248	SSAMSAAKAIADHIR	acetylation	[3]
298	VIKNKTWKFVEGLPI	acetylation	[1, 3, 4, 5, 6]
298	VIKNKTWKFVEGLPI	succinylation	[5]
298	VIKNKTWKFVEGLPI	ubiquitination	[2]
312	INDFSREKMDLTAKE	ubiquitination	[2]
318	EKMDLTAKELTEEKE	acetylation	[5]
318	EKMDLTAKELTEEKE	succinylation	[5]
318	EKMDLTAKELTEEKE	ubiquitination	[2]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [6] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 

Functional Description

  

Sequence Annotation

 NP_BIND 11 17 NAD (By similarity).
 NP_BIND 129 131 NAD (By similarity).
 ACT_SITE 187 187 Proton acceptor (By similarity).
 BINDING 92 92 Substrate (By similarity).
 BINDING 98 98 Substrate (By similarity).
 BINDING 105 105 NAD (By similarity).
 BINDING 112 112 NAD (By similarity).
 BINDING 131 131 Substrate (By similarity).
 BINDING 162 162 Substrate (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 118 118 N6-acetyllysine (By similarity).
 MOD_RES 121 121 N6-acetyllysine (By similarity).
 MOD_RES 210 210 Phosphotyrosine.
 MOD_RES 241 241 Phosphoserine.
 MOD_RES 298 298 N6-acetyllysine (By similarity).
 MOD_RES 333 333 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Tricarboxylic acid cycle; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 334 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0030060; F:L-malate dehydrogenase activity; IEA:EC.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:Compara.
 GO:0006734; P:NADH metabolic process; IEA:Compara.
 GO:0006107; P:oxaloacetate metabolic process; IEA:Compara.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR001252; Malate_DH_AS.
 IPR011274; Malate_DH_NAD-dep_euk.
 IPR010945; Malate_DH_type2.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00068; MDH 

PRINTS