CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006169
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell wall assembly regulator SMI1 
Protein Synonyms/Alias
 Killer toxin-resistance protein 4 
Gene Name
 SMI1 
Gene Synonyms/Alias
 KNR4; KTR4; YGR229C; G8553 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
36TPTFNMGKRLNSNNGubiquitination[1]
349NLRSQQQKSQPDTSLubiquitination[1]
360DTSLQEQKYVPASQKubiquitination[1]
383TLNAESIKGEDSGSAubiquitination[1]
405HESVKIVKTEPSEAEubiquitination[1]
436KADNVDIKQESERKEubiquitination[1]
453KQPKVEEKEHVENEHubiquitination[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Protein involved in the regulation of cell wall assembly and 1,3-beta-glucan synthesis, possibly through the transcriptional regulation of cell wall glucan and chitin synthesis. 
Sequence Annotation
 MOD_RES 202 202 Phosphoserine.
 MOD_RES 203 203 Phosphoserine.
 MOD_RES 376 376 Phosphothreonine.
 MOD_RES 381 381 Phosphoserine.
 MOD_RES 394 394 Phosphoserine.
 MOD_RES 400 400 Phosphoserine.  
Keyword
 Cell wall biogenesis/degradation; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 505 AA 
Protein Sequence
MDLFKRKVKE WVYSLSTDDH YAEYNPDETP TFNMGKRLNS NNGQVNPSQM HLNSVDEEMS 60
MGFQNGVPSN EDINIDEFTS TESNDGVSET LLAWRHIDFW TSEHNPDLNA TLSDPCTQND 120
ITHAEEDLEV SFPNPVKASF KIHDGQEDLE SMTGTSGLFY GFQLMTLDQV VAMTQAWRNV 180
AKNLNKRSQQ GLSHVTSTGS SSSMERLNGN KFKLPNIPDQ KSIPPNAVQP VYAHPAWIPL 240
ITDNAGNHIG VDLAPGPNGK YAQIITFGRD FDTKFVIAEN WGEFLLSFAN DLEAGNWYLV 300
DDNDDYFSGD GELVFRDKKS NGPIQDYFEV LKRRTWIKYQ ENLRSQQQKS QPDTSLQEQK 360
YVPASQKKVA AEQPSTLNAE SIKGEDSGSA DVQSVQDHES VKIVKTEPSE AETTTVNTES 420
LGQAEHEIKA DNVDIKQESE RKEDEKQPKV EEKEHVENEH VTESAKKDDD VNKQTEEMNK 480
KEENEIRSDD AKVEEAREEF ENIAL 505 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0000131; C:incipient cellular bud site; IDA:SGD.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0042546; P:cell wall biogenesis; IEA:InterPro.
 GO:0032995; P:regulation of fungal-type cell wall biogenesis; IMP:SGD.
 GO:0007346; P:regulation of mitotic cell cycle; IMP:SGD.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR009203; Cell_wall_assmbl/prolif_KNR4.
 IPR018958; Cell_wall_assmbl_KNR4-like. 
Pfam
 PF09346; SMI1_KNR4 
SMART
 SM00860; SMI1_KNR4 
PROSITE
  
PRINTS