CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006980
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Asparagine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Asparaginyl-tRNA synthetase; AsnRS 
Gene Name
 DED81 
Gene Synonyms/Alias
 YHR019C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
38ALFASQQKSDATEPKubiquitination[1]
45KSDATEPKLFVFKTEacetylation[2]
65EISASALKKARKGCDacetylation[2]
127RIYDSYSKVGQRVKVubiquitination[1]
388KQLNPNFKAPKAPFMacetylation[2]
388KQLNPNFKAPKAPFMubiquitination[1]
451TRFPVEIKSFYMKRCacetylation[2]
496DELMAGFKREGIDTDacetylation[2]
496DELMAGFKREGIDTDubiquitination[1]
513YWFIDQRKYGTCPHGacetylation[2]
513YWFIDQRKYGTCPHGubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
  
Sequence Annotation
  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Nucleotide-binding; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 554 AA 
Protein Sequence
MSSLYIKEAT GVDELTTAGS QDHPFKTPAY ALFASQQKSD ATEPKLFVFK TEDNEYQEIS 60
ASALKKARKG CDGLKKKAVK QKEQELKKQQ KEAENAAKQL SALNITIKED ESLPAAIKTR 120
IYDSYSKVGQ RVKVSGWIHR LRSNKKVIFV VLRDGSGFIQ CVLSGDLALA QQTLDLTLES 180
TVTLYGTIVK LPEGKTAPGG VELNVDYYEV VGLAPGGEDS FTNKIAEGSD PSLLLDQRHL 240
ALRGDALSAV MKVRAALLKS VRRVYDEEHL TEVTPPCMVQ TQVEGGSTLF KMNYYGEEAY 300
LTQSSQLYLE TCLASLGDVY TIQESFRAEK SHTRRHLSEY THIEAELAFL TFDDLLQHIE 360
TLIVKSVQYV LEDPIAGPLV KQLNPNFKAP KAPFMRLQYK DAITWLNEHD IKNEEGEDFK 420
FGDDIAEAAE RKMTDTIGVP IFLTRFPVEI KSFYMKRCSD DPRVTESVDV LMPNVGEITG 480
GSMRIDDMDE LMAGFKREGI DTDAYYWFID QRKYGTCPHG GYGIGTERIL AWLCDRFTVR 540
DCSLYPRFSG RCKP 554 
Gene Ontology
 GO:0005829; C:cytosol; IC:SGD.
 GO:0004816; F:asparagine-tRNA ligase activity; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006421; P:asparaginyl-tRNA aminoacylation; IGI:SGD. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR004522; Asn-tRNA-ligase_IIb.
 IPR002312; Asp/Asn-tRNA-synth_IIb.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 
Pfam
 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01042; TRNASYNTHASP.