UniProt Accession

 ULK3_HUMAN; Q6PHR2; B2RXK3; B4DRQ7; D3DW68; Q9NPN5; Q9UFS4 

Genbank Protein ID

 AK299380; AL117482; AC091230; BC036117; BC056423; CH471136; CH471136; BC157884; AL360256 

Genbank Nucleotide ID

 BAG61369.1; CAB55955.2; EAW99301.1; EAW99303.1; AAI57885.1; CAB96176.1 

Protein Name

 Serine/threonine-protein kinase ULK3 

Protein Synonyms/Alias

 Unc-51-like kinase 3 

Gene Name

 ULK3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
53	VAKKSLNKASVENLL	ubiquitination	[1]
67	LTEIEILKGIRHPHI	ubiquitination	[1]
139	NISHLDLKPQNILLS	ubiquitination	[1]
170	HMSPWDEKHVLRGSP	ubiquitination	[1]
224	SFSELEEKIRSNRVI	ubiquitination	[1]
294	LVVQAVKKDQEGDSA	ubiquitination	[1]
333	RKEAIKAKVGQYVSR	ubiquitination	[1]
345	VSRAEELKAIVSSSN	ubiquitination	[1]
441	MARAEYLKEQMRESR	ubiquitination	[1]
456	WEADTLDKEGLSESV	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy. 

Sequence Annotation

 DOMAIN 14 270 Protein kinase.
 DOMAIN 280 348 MIT 1.
 DOMAIN 375 444 MIT 2.
 NP_BIND 20 28 ATP (By similarity).
 ACT_SITE 137 137 Proton acceptor (By similarity).
 BINDING 44 44 ATP (By similarity).
 MOD_RES 176 176 Phosphoserine.
 MOD_RES 300 300 Phosphoserine; by autocatalysis.
 MOD_RES 350 350 Phosphoserine; by autocatalysis.
 MOD_RES 384 384 Phosphoserine; by autocatalysis.
 MOD_RES 464 464 Phosphoserine; by autocatalysis.  

Keyword

 Alternative splicing; ATP-binding; Autophagy; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 472 AA 

Protein Sequence

Gene Ontology

 GO:0000407; C:pre-autophagosomal structure; TAS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0006914; P:autophagy; TAS:UniProtKB.
 GO:0090398; P:cellular senescence; TAS:UniProtKB.
 GO:0045879; P:negative regulation of smoothened signaling pathway; IDA:UniProtKB.
 GO:0045880; P:positive regulation of smoothened signaling pathway; IDA:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR007330; MIT.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF04212; MIT
 PF00069; Pkinase 

SMART

 SM00745; MIT
 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS