UniProt Accession

 ARO1_YEAST; P08566; D6VSB3 

Genbank Protein ID

 X06077; Z48179; X13802; X13803; BK006938 

Genbank Nucleotide ID

 CAA29458.1; CAA88208.1; CAA32036.1; CAA32037.1; DAA11973.1 

Protein Name

 Pentafunctional AROM polypeptide 

Protein Synonyms/Alias

 3-dehydroquinate synthase; DHQS; 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; Shikimate kinase; SK; 3-dehydroquinate dehydratase; 3-dehydroquinase; Shikimate dehydrogenase 

Gene Name

 ARO1 

Gene Synonyms/Alias

 AROM; YDR127W; YD9302.02 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
75	RLLTYVVKPGETSKS	ubiquitination	[1]
180	KWLETLAKREFINGM	ubiquitination	[1]
258	VLESIKVKAEVVSSD	ubiquitination	[1]
305	CVSIGMVKEAELSRY	ubiquitination	[1]
326	TQVARLSKILVAYGL	acetylation	[2]
340	LPVSPDEKWFKELTL	acetylation	[2]
343	SPDEKWFKELTLHKK	acetylation	[2]
358	TPLDILLKKMSIDKK	acetylation	[2]
425	VVIPPGSKSISNRAL	ubiquitination	[1]
454	LLHSDDTKHMLTAVH	acetylation	[2]
695	RFARDVLKPMGCKIT	ubiquitination	[1]
779	AMATELAKFGVKTTE	ubiquitination	[1]
803	LNSIKDLKVPSDSSG	ubiquitination	[1]
933	QHNNQSVKQFVVENG	ubiquitination	[1]
1058	ALRRSFSKYIATITG	ubiquitination	[1]
1127	KQLSILRKATDSIPI	ubiquitination	[1]
1337	EILGLPHKFDKFETE	acetylation	[2]
1340	GLPHKFDKFETESAQ	ubiquitination	[1]
1350	TESAQLVKEKLLDGN	ubiquitination	[1]
1402	PLGNKKFKGDNTDWL	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis. 

Sequence Annotation

 NP_BIND 43 45 NAD (By similarity).
 NP_BIND 78 81 NAD (By similarity).
 NP_BIND 109 111 NAD (By similarity).
 NP_BIND 134 135 NAD (By similarity).
 NP_BIND 174 177 NAD (By similarity).
 NP_BIND 895 902 ATP (By similarity).
 REGION 1 392 3-dehydroquinate synthase.
 REGION 189 192 Substrate binding 2 (By similarity).
 REGION 272 276 Substrate binding 2 (By similarity).
 REGION 405 871 EPSP synthase.
 REGION 890 1080 Shikimate kinase.
 REGION 1081 1293 3-dehydroquinase.
 REGION 1306 1588 Shikimate dehydrogenase.
 ACT_SITE 268 268 Proton acceptor; for 3-dehydroquinate
 ACT_SITE 283 283 Proton acceptor; for 3-dehydroquinate
 ACT_SITE 853 853 For EPSP synthase activity (Potential).
 ACT_SITE 1198 1198 Proton acceptor; for 3-dehydroquinate
 ACT_SITE 1227 1227 Schiff-base intermediate with substrate;
 METAL 189 189 Zinc; catalytic (By similarity).
 METAL 279 279 Zinc; catalytic (By similarity).
 METAL 295 295 Zinc; catalytic (By similarity).
 BINDING 114 114 NAD (By similarity).
 BINDING 125 125 Substrate 1 (By similarity).
 BINDING 141 141 Substrate 2 (By similarity).
 BINDING 147 147 Substrate 2 (By similarity).
 BINDING 156 156 NAD (By similarity).
 BINDING 157 157 Substrate 2 (By similarity).
 BINDING 185 185 NAD (By similarity).
 BINDING 258 258 Substrate 2 (By similarity).
 BINDING 279 279 Substrate 2 (By similarity).
 BINDING 295 295 Substrate 2 (By similarity).
 BINDING 364 364 Substrate 2 (By similarity).  

Keyword

 Amino-acid biosynthesis; Aromatic amino acid biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Kinase; Lyase; Metal-binding; Multifunctional enzyme; NADP; Nucleotide-binding; Oxidoreductase; Reference proteome; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1588 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0003855; F:3-dehydroquinate dehydratase activity; IDA:SGD.
 GO:0003856; F:3-dehydroquinate synthase activity; IDA:SGD.
 GO:0003866; F:3-phosphoshikimate 1-carboxyvinyltransferase activity; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004764; F:shikimate 3-dehydrogenase (NADP+) activity; IDA:SGD.
 GO:0004765; F:shikimate kinase activity; IDA:SGD.
 GO:0009073; P:aromatic amino acid family biosynthetic process; IEA:UniProtKB-KW.
 GO:0009423; P:chorismate biosynthetic process; TAS:SGD. 

Interpro

 IPR018508; 3-dehydroquinate_DH_AS.
 IPR013785; Aldolase_TIM.
 IPR016037; DHQ_synth_AroB.
 IPR001381; DHquinase_I.
 IPR001986; Enolpyruvate_Tfrase_dom.
 IPR006264; EPSP_synthase.
 IPR023193; EPSP_synthase_CS.
 IPR016040; NAD(P)-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR008289; Pentafunct_AroM.
 IPR013792; RNA3'P_cycl/enolpyr_Trfase_a/b.
 IPR013708; Shikimate_DH-bd_N.
 IPR010110; Shikimate_DH_AroM-type.
 IPR000623; Shikimate_kinase/TSH1.
 IPR023000; Shikimate_kinase_CS.
 IPR006151; Shikm_DH/Glu-tRNA_Rdtase. 

Pfam

 PF01487; DHquinase_I
 PF00275; EPSP_synthase
 PF01488; Shikimate_DH
 PF08501; Shikimate_dh_N
 PF01202; SKI 

SMART

  

PROSITE

 PS01028; DEHYDROQUINASE_I
 PS00104; EPSP_SYNTHASE_1
 PS00885; EPSP_SYNTHASE_2
 PS01128; SHIKIMATE_KINASE 

PRINTS

 PR01100; SHIKIMTKNASE.