CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020096
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 9 
Protein Synonyms/Alias
 Rho-type GTPase-activating protein 9 
Gene Name
 ARHGAP9 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
56DRFLLLRKTNSDWWLubiquitination[1]
264MEGTQTLKRNNDVLQubiquitination[1]
275DVLQPQAKGFRSDTGubiquitination[1]
333SGLLNMTKIAQGGRKubiquitination[1]
489EESELVSKPLLRLSSubiquitination[1]
522KLKRLIAKRPPLQSLubiquitination[1]
568LCIAAVDKRGLDVDGubiquitination[1]
588GNLAVVQKLRFLVDRubiquitination[1, 2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. 
Sequence Annotation
 DOMAIN 22 88 SH3.
 DOMAIN 213 247 WW.
 DOMAIN 322 435 PH.
 DOMAIN 542 749 Rho-GAP.
 MOD_RES 475 475 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; GTPase activation; Phosphoprotein; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 750 AA 
Protein Sequence
MLSSRWWPSS WGILGLGPRS PPRGSQLCAL YAFTYTGADG QQVSLAEGDR FLLLRKTNSD 60
WWLARRLEAP STSRPIFVPA AYMIEESIPS QSPTTVIPGQ LLWTPGPKLF HGSLEELSQA 120
LPSRAQASSE QPPPLPRKMC RSVSTDNLSP SLLKPFQEGP SGRSLSQEDL PSEASASTAG 180
PQPLMSEPPV YCNLVDLRRC PRSPPPGPAC PLLQRLDAWE QHLDPNSGRC FYINSLTGCK 240
SWKPPRRSRS ETNPGSMEGT QTLKRNNDVL QPQAKGFRSD TGTPEPLDPQ GSLSLSQRTS 300
QLDPPALQAP RPLPQLLDDP HEVEKSGLLN MTKIAQGGRK LRKNWGPSWV VLTGNSLVFY 360
REPPPTAPSS GWGPAGSRPE SSVDLRGAAL AHGRHLSSRR NVLHIRTIPG HEFLLQSDHE 420
TELRAWHRAL RTVIERLVRW VEARREAPTG RDQGSGDREN PLELRLSGSG PAELSAGEDE 480
EEESELVSKP LLRLSSRRSS IRGPEGTEQN RVRNKLKRLI AKRPPLQSLQ ERGLLRDQVF 540
GCQLESLCQR EGDTVPSFLR LCIAAVDKRG LDVDGIYRVS GNLAVVQKLR FLVDRERAVT 600
SDGRYVFPEQ PGQEGRLDLD STEWDDIHVV TGALKLFLRE LPQPLVPPLL LPHFRAALAL 660
SESEQCLSQI QELIGSMPKP NHDTLRYLLE HLCRVIAHSD KNRMTPHNLG IVFGPTLFRP 720
EQETSDPAAH ALYPGQLVQL MLTNFTSLFP 750 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005096; F:GTPase activator activity; IDA:MGI.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR015767; Rho_GTPase_activating.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR001452; SH3_domain.
 IPR001202; WW_dom. 
Pfam
 PF00169; PH
 PF00620; RhoGAP
 PF00018; SH3_1 
SMART
 SM00233; PH
 SM00324; RhoGAP
 SM00326; SH3 
PROSITE
 PS50003; PH_DOMAIN
 PS50238; RHOGAP
 PS50002; SH3
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS