CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016809
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dual specificity mitogen-activated protein kinase kinase 7 
Protein Synonyms/Alias
 MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; c-Jun N-terminal kinase kinase 2; JNK kinase 2; JNKK 2 
Gene Name
 Map2k7 
Gene Synonyms/Alias
 Mkk7 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
9AASSLEQKLSRLEAKubiquitination[1]
342QFPYKNCKTDFEVLTubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. 
Sequence Annotation
 DOMAIN 136 396 Protein kinase.
 NP_BIND 142 150 ATP (By similarity).
 REGION 37 73 D domain (By similarity).
 REGION 393 416 DVD domain (By similarity).
 ACT_SITE 259 259 Proton acceptor (By similarity).
 BINDING 165 165 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 287 287 Phosphoserine; by MAP3K (By similarity).
 MOD_RES 291 291 Phosphothreonine; by MAP3K (By  
Keyword
 Acetylation; Alternative splicing; Apoptosis; ATP-binding; Coiled coil; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Stress response; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 535 AA 
Protein Sequence
MAASSLEQKL SRLEAKLKQE NREARRRIDL NLDISPQRPR PIIVITLSPA PAPSQRAALQ 60
LPLANDGGSR SPSSESSPQH PTPPTRPRHM LGLPSTLFTP RSMESIEIDQ KLQEIMKQTG 120
YLTIGGQRYQ AEINDLENLG EMGSGTCGQV WKMRFRKTGH IIAVKQMRRS GNKEENKRIL 180
MDLDVVLKSH DCPYIVQCFG TFITNTDVFI AMELMGTCAE KLKKRMQGPI PERILGKMTV 240
AIVKALYYLK EKHGVIHRDV KPSNILLDER GQIKLCDFGI SGRLVDSKAK TRSAGCAAYM 300
APERIDPPDP TKPDYDIRAD VWSLGISLVE LATGQFPYKN CKTDFEVLTK VLQEEPPLLP 360
GHMGFSGDFQ SFVKDCLTKD HRKRPKYNKL LEHSFIKHYE ILEVDVASWF KDVMAKTESP 420
RTSGVLSQHH LPFFSTSVTW GAWPLAAQTP FQSGVIRCRG RVPSPRRATG GSGGQPCVCA 480
GGPGPSFTEM GPSPSPMLSN TFFTPDPGAC PGASTWGLPR RRLCQLLTTS TPGCC 535 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008545; F:JUN kinase kinase activity; IEA:Compara.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004708; F:MAP kinase kinase activity; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004713; F:protein tyrosine kinase activity; IEA:UniProtKB-KW.
 GO:0007257; P:activation of JUN kinase activity; IDA:BHF-UCL.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS