UniProt Accession

 DYRL1_HUMAN; Q86XF0; D3DN30; Q6P4I9 

Genbank Protein ID

 AL832912; CH471052; CH471052; BC045541; BC063379 

Genbank Nucleotide ID

 CAH10617.1; EAW79895.1; EAW79896.1; AAH45541.1; AAH63379.1 

Protein Name

 Dihydrofolate reductase, mitochondrial 

Protein Synonyms/Alias

 Dihydrofolate reductase-like protein 1 

Gene Name

 DHFRL1 

Gene Synonyms/Alias

 DHFRP4 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	NLVIMGRKTWFSIPE	ubiquitination	[1]
179	EGKHIKYKFEVCEKD	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Binds its own mRNA and that of DHFR. 

Sequence Annotation

 DOMAIN 4 185 DHFR.
 NP_BIND 16 22 NADP (By similarity).
 NP_BIND 55 57 NADP (By similarity).
 NP_BIND 77 79 NADP (By similarity).
 NP_BIND 117 124 NADP (By similarity).
 REGION 31 36 Substrate binding (By similarity).
 BINDING 10 10 NADP; via amide nitrogen and carbonyl
 BINDING 71 71 Substrate (By similarity).  

Keyword

 Complete proteome; Membrane; Mitochondrion; Mitochondrion inner membrane; NADP; One-carbon metabolism; Oxidoreductase; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 187 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IMP:UniProtKB.
 GO:0005759; C:mitochondrial matrix; IMP:UniProtKB.
 GO:0004146; F:dihydrofolate reductase activity; IMP:UniProtKB.
 GO:0003729; F:mRNA binding; IDA:UniProtKB.
 GO:0050661; F:NADP binding; IEA:InterPro.
 GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
 GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
 GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
 GO:0046654; P:tetrahydrofolate biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0046653; P:tetrahydrofolate metabolic process; IDA:UniProtKB.
 GO:0046105; P:thymidine biosynthetic process; IDA:UniProtKB. 

Interpro

 IPR012259; DHFR.
 IPR024072; DHFR-like_dom.
 IPR001796; DHFR_dom. 

Pfam

 PF00186; DHFR_1 

SMART

  

PROSITE

 PS00075; DHFR_1
 PS51330; DHFR_2 

PRINTS

 PR00070; DHFR.