CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004435
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Beta-galactosidase 
Protein Synonyms/Alias
 Acid beta-galactosidase; Lactase; Elastin receptor 1 
Gene Name
 GLB1 
Gene Synonyms/Alias
 ELNR1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43YSRDSFLKDGQPFRYubiquitination[1]
225FTTDGAHKTFLKCGAubiquitination[2]
383KVTLEKLKTVGAALDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. 
Sequence Annotation
 ACT_SITE 188 188 Proton donor (Potential).
 ACT_SITE 268 268 Nucleophile (Potential).
 CARBOHYD 26 26 N-linked (GlcNAc...) (Potential).
 CARBOHYD 247 247 N-linked (GlcNAc...) (Potential).
 CARBOHYD 464 464 N-linked (GlcNAc...).
 CARBOHYD 498 498 N-linked (GlcNAc...) (Potential).
 CARBOHYD 542 542 N-linked (GlcNAc...) (Potential).
 CARBOHYD 545 545 N-linked (GlcNAc...) (Potential).
 CARBOHYD 555 555 N-linked (GlcNAc...).  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Gangliosidosis; Glycoprotein; Glycosidase; Hydrolase; Lysosome; Mucopolysaccharidosis; Polymorphism; Reference proteome; Signal; Zymogen. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 677 AA 
Protein Sequence
MPGFLVRILP LLLVLLLLGP TRGLRNATQR MFEIDYSRDS FLKDGQPFRY ISGSIHYSRV 60
PRFYWKDRLL KMKMAGLNAI QTYVPWNFHE PWPGQYQFSE DHDVEYFLRL AHELGLLVIL 120
RPGPYICAEW EMGGLPAWLL EKESILLRSS DPDYLAAVDK WLGVLLPKMK PLLYQNGGPV 180
ITVQVENEYG SYFACDFDYL RFLQKRFRHH LGDDVVLFTT DGAHKTFLKC GALQGLYTTV 240
DFGTGSNITD AFLSQRKCEP KGPLINSEFY TGWLDHWGQP HSTIKTEAVA SSLYDILARG 300
ASVNLYMFIG GTNFAYWNGA NSPYAAQPTS YDYDAPLSEA GDLTEKYFAL RNIIQKFEKV 360
PEGPIPPSTP KFAYGKVTLE KLKTVGAALD ILCPSGPIKS LYPLTFIQVK QHYGFVLYRT 420
TLPQDCSNPA PLSSPLNGVH DRAYVAVDGI PQGVLERNNV ITLNITGKAG ATLDLLVENM 480
GRVNYGAYIN DFKGLVSNLT LSSNILTDWT IFPLDTEDAV RSHLGGWGHR DSGHHDEAWA 540
HNSSNYTLPA FYMGNFSIPS GIPDLPQDTF IQFPGWTKGQ VWINGFNLGR YWPARGPQLT 600
LFVPQHILMT SAPNTITVLE LEWAPCSSDD PELCAVTFVD RPVIGSSVTY DHPSKPVEKR 660
LMPPPPQKNK DSWLDHV 677 
Gene Ontology
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004565; F:beta-galactosidase activity; TAS:UniProtKB.
 GO:0016936; F:galactoside binding; IEA:Compara.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019388; P:galactose catabolic process; IEA:Compara.
 GO:0006687; P:glycosphingolipid metabolic process; TAS:Reactome.
 GO:0042340; P:keratan sulfate catabolic process; TAS:Reactome. 
Interpro
 IPR026283; B-gal_1-like.
 IPR008979; Galactose-bd-like.
 IPR019801; Glyco_hydro_35_CS.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR001944; Glycoside_Hdrlase_35.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF01301; Glyco_hydro_35 
SMART
  
PROSITE
 PS01182; GLYCOSYL_HYDROL_F35 
PRINTS
 PR00742; GLHYDRLASE35.