CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005790
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nitric oxide synthase, brain 
Protein Synonyms/Alias
 Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS 
Gene Name
 NOS1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
24SVRLFKRKVGGLGFLacetylation[1]
33GGLGFLVKERVSKPPacetylation[1]
38LVKERVSKPPVIISDacetylation[1]
131QPLGPPTKAVDLSHQacetylation[1]
234APAKAEMKDMGIQVDacetylation[1]
250DLDGKSHKPLPLGVEacetylation[1]
356RPEDVRTKGQLFPLAubiquitination[2]
759QAMAKRVKATILYATacetylation[1]
861SSYSDSQKSSGDGPDacetylation[1]
994QGLSNVHKKRVSAARacetylation[1]
1305REETLQAKNKGVFREacetylation[1]
1326REPDKPKKYVQDILQacetylation[1]
1376RIMTQQGKLSAEDAGacetylation[1]
Reference
 [1] Calreticulin transacetylase mediates the acetylation of nitric oxide synthase by polyphenolic acetate.
 Bansal S, Gaspari M, Raj HG, Kumar A, Cuda G, Verheij E, Tyagi YK, Ponnan P, Rastogi RC, Parmar VS.
 Appl Biochem Biotechnol. 2008 Jan;144(1):37-45. [PMID: 18415985]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. 
Sequence Annotation
 DOMAIN 17 99 PDZ.
 DOMAIN 760 940 Flavodoxin-like.
 DOMAIN 995 1242 FAD-binding FR-type.
 NP_BIND 886 917 FMN (By similarity).
 NP_BIND 1032 1043 FAD (By similarity).
 NP_BIND 1175 1185 FAD (By similarity).
 NP_BIND 1250 1268 NADP (By similarity).
 NP_BIND 1348 1363 NADP (By similarity).
 REGION 1 205 Interaction with NOSIP (By similarity).
 REGION 163 245 PIN (nNOS-inhibiting protein) binding.
 REGION 730 750 Calmodulin-binding (Potential).
 REGION 755 774 Tetrahydrobiopterin-binding (By
 METAL 420 420 Iron (heme axial ligand) (By similarity).  
Keyword
 Alternative splicing; Calmodulin-binding; Cell membrane; Cell projection; Complete proteome; FAD; FMN; Heme; Iron; Membrane; Metal-binding; NADP; Oxidoreductase; Polymorphism; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1434 AA 
Protein Sequence
MEDHMFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG AAEQSGLIQA 60
GDIILAVNGR PLVDLSYDSA LEVLRGIASE THVVLILRGP EGFTTHLETT FTGDGTPKTI 120
RVTQPLGPPT KAVDLSHQPP AGKEQPLAVD GASGPGNGPQ HAYDDGQEAG SLPHANGLAP 180
RPPGQDPAKK ATRVSLQGRG ENNELLKEIE PVLSLLTSGS RGVKGGAPAK AEMKDMGIQV 240
DRDLDGKSHK PLPLGVENDR VFNDLWGKGN VPVVLNNPYS EKEQPPTSGK QSPTKNGSPS 300
KCPRFLKVKN WETEVVLTDT LHLKSTLETG CTEYICMGSI MHPSQHARRP EDVRTKGQLF 360
PLAKEFIDQY YSSIKRFGSK AHMERLEEVN KEIDTTSTYQ LKDTELIYGA KHAWRNASRC 420
VGRIQWSKLQ VFDARDCTTA HGMFNYICNH VKYATNKGNL RSAITIFPQR TDGKHDFRVW 480
NSQLIRYAGY KQPDGSTLGD PANVQFTEIC IQQGWKPPRG RFDVLPLLLQ ANGNDPELFQ 540
IPPELVLEVP IRHPKFEWFK DLGLKWYGLP AVSNMLLEIG GLEFSACPFS GWYMGTEIGV 600
RDYCDNSRYN ILEEVAKKMN LDMRKTSSLW KDQALVEINI AVLYSFQSDK VTIVDHHSAT 660
ESFIKHMENE YRCRGGCPAD WVWIVPPMSG SITPVFHQEM LNYRLTPSFE YQPDPWNTHV 720
WKGTNGTPTK RRAIGFKKLA EAVKFSAKLM GQAMAKRVKA TILYATETGK SQAYAKTLCE 780
IFKHAFDAKV MSMEEYDIVH LEHETLVLVV TSTFGNGDPP ENGEKFGCAL MEMRHPNSVQ 840
EERKSYKVRF NSVSSYSDSQ KSSGDGPDLR DNFESAGPLA NVRFSVFGLG SRAYPHFCAF 900
GHAVDTLLEE LGGERILKMR EGDELCGQEE AFRTWAKKVF KAACDVFCVG DDVNIEKANN 960
SLISNDRSWK RNKFRLTFVA EAPELTQGLS NVHKKRVSAA RLLSRQNLQS PKSSRSTIFV 1020
RLHTNGSQEL QYQPGDHLGV FPGNHEDLVN ALIERLEDAP PVNQMVKVEL LEERNTALGV 1080
ISNWTDELRL PPCTIFQAFK YYLDITTPPT PLQLQQFASL ATSEKEKQRL LVLSKGLQEY 1140
EEWKWGKNPT IVEVLEEFPS IQMPATLLLT QLSLLQPRYY SISSSPDMYP DEVHLTVAIV 1200
SYRTRDGEGP IHHGVCSSWL NRIQADELVP CFVRGAPSFH LPRNPQVPCI LVGPGTGIAP 1260
FRSFWQQRQF DIQHKGMNPC PMVLVFGCRQ SKIDHIYREE TLQAKNKGVF RELYTAYSRE 1320
PDKPKKYVQD ILQEQLAESV YRALKEQGGH IYVCGDVTMA ADVLKAIQRI MTQQGKLSAE 1380
DAGVFISRMR DDNRYHEDIF GVTLRTYEVT NRLRSESIAF IEESKKDTDE VFSS 1434 
Gene Ontology
 GO:0005856; C:cytoskeleton; ISS:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043197; C:dendritic spine; IEA:UniProtKB-SubCell.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:BHF-UCL.
 GO:0001917; C:photoreceptor inner segment; ISS:BHF-UCL.
 GO:0042383; C:sarcolemma; IDA:BHF-UCL.
 GO:0016529; C:sarcoplasmic reticulum; IDA:BHF-UCL.
 GO:0045202; C:synapse; IEA:Compara.
 GO:0034618; F:arginine binding; TAS:BHF-UCL.
 GO:0046870; F:cadmium ion binding; ISS:BHF-UCL.
 GO:0050660; F:flavin adenine dinucleotide binding; ISS:BHF-UCL.
 GO:0010181; F:FMN binding; ISS:BHF-UCL.
 GO:0020037; F:heme binding; ISS:BHF-UCL.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0050661; F:NADP binding; ISS:BHF-UCL.
 GO:0003958; F:NADPH-hemoprotein reductase activity; IBA:RefGenome.
 GO:0004517; F:nitric-oxide synthase activity; ISS:BHF-UCL.
 GO:0034617; F:tetrahydrobiopterin binding; NAS:BHF-UCL.
 GO:0006527; P:arginine catabolic process; IC:BHF-UCL.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0071363; P:cellular response to growth factor stimulus; IEA:Compara.
 GO:0042738; P:exogenous drug catabolic process; IEA:Compara.
 GO:0051701; P:interaction with host; TAS:Reactome.
 GO:0033555; P:multicellular organismal response to stress; IMP:BHF-UCL.
 GO:0007520; P:myoblast fusion; TAS:BHF-UCL.
 GO:0045776; P:negative regulation of blood pressure; IBA:RefGenome.
 GO:0010523; P:negative regulation of calcium ion transport into cytosol; TAS:BHF-UCL.
 GO:0051346; P:negative regulation of hydrolase activity; IEA:Compara.
 GO:0043267; P:negative regulation of potassium ion transport; IEA:Compara.
 GO:0051612; P:negative regulation of serotonin uptake; IEA:Compara.
 GO:0042136; P:neurotransmitter biosynthetic process; TAS:BHF-UCL.
 GO:0006809; P:nitric oxide biosynthetic process; ISS:BHF-UCL.
 GO:0007263; P:nitric oxide mediated signal transduction; IBA:RefGenome.
 GO:0018119; P:peptidyl-cysteine S-nitrosylation; IEA:Compara.
 GO:0090382; P:phagosome maturation; TAS:Reactome.
 GO:0031284; P:positive regulation of guanylate cyclase activity; IBA:RefGenome.
 GO:0035066; P:positive regulation of histone acetylation; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0045909; P:positive regulation of vasodilation; IDA:BHF-UCL.
 GO:0055117; P:regulation of cardiac muscle contraction; TAS:BHF-UCL.
 GO:0002028; P:regulation of sodium ion transport; IEA:Compara.
 GO:0009408; P:response to heat; IDA:BHF-UCL.
 GO:0001666; P:response to hypoxia; IEP:BHF-UCL.
 GO:0006941; P:striated muscle contraction; IEA:Compara. 
Interpro
 IPR003097; FAD-binding_1.
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001094; Flavdoxin.
 IPR008254; Flavodoxin/NO_synth.
 IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
 IPR023173; NADPH_Cyt_P450_Rdtase_dom3.
 IPR004030; NO_synthase_oxygenase_dom.
 IPR026009; NOS.
 IPR012144; NOS_met.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR001478; PDZ.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00667; FAD_binding_1
 PF00258; Flavodoxin_1
 PF00175; NAD_binding_1
 PF02898; NO_synthase
 PF00595; PDZ 
SMART
 SM00228; PDZ 
PROSITE
 PS51384; FAD_FR
 PS50902; FLAVODOXIN_LIKE
 PS60001; NOS
 PS50106; PDZ 
PRINTS
 PR00369; FLAVODOXIN.
 PR00371; FPNCR.