CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002111
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HLA class II histocompatibility antigen gamma chain 
Protein Synonyms/Alias
 HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; CD74 
Gene Name
 CD74 
Gene Synonyms/Alias
 DHLAG 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43RPGAPESKCSRGALYubiquitination[1]
80QQQGRLDKLTVTSQNubiquitination[1]
159LKVYPPLKGSFPENLubiquitination[2, 3]
170PENLRHLKNTMETIDubiquitination[2, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF. 
Sequence Annotation
 DOMAIN 210 271 Thyroglobulin type-1.
 REGION 103 117 CLIP.
 MOD_RES 25 25 Phosphoserine (By similarity).
 CARBOHYD 130 130 N-linked (GlcNAc...).
 CARBOHYD 136 136 N-linked (GlcNAc...).
 CARBOHYD 203 203 O-linked (GalNAc...).
 CARBOHYD 256 256 N-linked (GlcNAc...).
 CARBOHYD 281 281 O-linked (GalNAc...).
 DISULFID 213 232
 DISULFID 243 250
 DISULFID 252 271  
Keyword
 3D-structure; Adaptive immunity; Alternative splicing; Cell membrane; Chaperone; Complete proteome; Direct protein sequencing; Disulfide bond; Endoplasmic reticulum; Endosome; Glycoprotein; Golgi apparatus; Immunity; Lysosome; Membrane; Phosphoprotein; Proteoglycan; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 296 AA 
Protein Sequence
MHRRRSRSCR EDQKPVMDDQ RDLISNNEQL PMLGRRPGAP ESKCSRGALY TGFSILVTLL 60
LAGQATTAYF LYQQQGRLDK LTVTSQNLQL ENLRMKLPKP PKPVSKMRMA TPLLMQALPM 120
GALPQGPMQN ATKYGNMTED HVMHLLQNAD PLKVYPPLKG SFPENLRHLK NTMETIDWKV 180
FESWMHHWLL FEMSRHSLEQ KPTDAPPKVL TKCQEEVSHI PAVHPGSFRP KCDENGNYLP 240
LQCYGSIGYC WCVFPNGTEV PNTRSRGHHN CSESLELEDP SSGLGVTKQD LGPVPM 296 
Gene Ontology
 GO:0009986; C:cell surface; IDA:BHF-UCL.
 GO:0030669; C:clathrin-coated endocytic vesicle membrane; TAS:Reactome.
 GO:0012507; C:ER to Golgi transport vesicle membrane; TAS:Reactome.
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0071556; C:integral to lumenal side of endoplasmic reticulum membrane; TAS:Reactome.
 GO:0043202; C:lysosomal lumen; TAS:Reactome.
 GO:0005765; C:lysosomal membrane; TAS:Reactome.
 GO:0035692; C:macrophage migration inhibitory factor receptor complex; IDA:BHF-UCL.
 GO:0042613; C:MHC class II protein complex; IEA:Compara.
 GO:0005771; C:multivesicular body; IEA:Compara.
 GO:0035693; C:NOS2-CD74 complex; IEA:Compara.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0032588; C:trans-Golgi network membrane; TAS:Reactome.
 GO:0001540; F:beta-amyloid binding; IPI:BHF-UCL.
 GO:0004896; F:cytokine receptor activity; IDA:BHF-UCL.
 GO:0042802; F:identical protein binding; TAS:UniProtKB.
 GO:0042289; F:MHC class II protein binding; NAS:UniProtKB.
 GO:0000187; P:activation of MAPK activity; IEA:Compara.
 GO:0019883; P:antigen processing and presentation of endogenous antigen; NAS:UniProtKB.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0008283; P:cell proliferation; IDA:UniProtKB.
 GO:0051085; P:chaperone mediated protein folding requiring cofactor; IEA:Compara.
 GO:0006952; P:defense response; IEA:Compara.
 GO:0016064; P:immunoglobulin mediated immune response; ISS:UniProtKB.
 GO:0006886; P:intracellular protein transport; ISS:UniProtKB.
 GO:0035691; P:macrophage migration inhibitory factor signaling pathway; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0043518; P:negative regulation of DNA damage response, signal transduction by p53 class mediator; IDA:BHF-UCL.
 GO:0002906; P:negative regulation of mature B cell apoptotic process; IEA:Compara.
 GO:0002792; P:negative regulation of peptide secretion; IDA:BHF-UCL.
 GO:0045581; P:negative regulation of T cell differentiation; IEA:Compara.
 GO:0045060; P:negative thymic T cell selection; IEA:Compara.
 GO:0030890; P:positive regulation of B cell proliferation; IMP:BHF-UCL.
 GO:2000343; P:positive regulation of chemokine (C-X-C motif) ligand 2 production; ISS:BHF-UCL.
 GO:0001961; P:positive regulation of cytokine-mediated signaling pathway; IMP:BHF-UCL.
 GO:0002606; P:positive regulation of dendritic cell antigen processing and presentation; IEA:Compara.
 GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:BHF-UCL.
 GO:0048146; P:positive regulation of fibroblast proliferation; IMP:BHF-UCL.
 GO:0060907; P:positive regulation of macrophage cytokine production; ISS:BHF-UCL.
 GO:0090023; P:positive regulation of neutrophil chemotaxis; ISS:BHF-UCL.
 GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:BHF-UCL.
 GO:0045582; P:positive regulation of T cell differentiation; IEA:Compara.
 GO:0002830; P:positive regulation of type 2 immune response; IEA:Compara.
 GO:0045059; P:positive thymic T cell selection; IEA:Compara.
 GO:0001516; P:prostaglandin biosynthetic process; IDA:UniProtKB.
 GO:0006461; P:protein complex assembly; ISS:UniProtKB.
 GO:0043030; P:regulation of macrophage activation; NAS:UniProtKB.
 GO:0045058; P:T cell selection; NAS:UniProtKB. 
Interpro
 IPR015386; MHC_II-assoc_invar/CLIP_MHC-bd.
 IPR022339; MHC_II-assoc_invar_chain.
 IPR011988; MHC_II-assoc_invariant_trimer.
 IPR000716; Thyroglobulin_1. 
Pfam
 PF09307; MHC2-interact
 PF08831; MHCassoc_trimer
 PF00086; Thyroglobulin_1 
SMART
 SM00211; TY 
PROSITE
 PS00484; THYROGLOBULIN_1_1
 PS51162; THYROGLOBULIN_1_2 
PRINTS
 PR01990; CD74ANTIGEN.