CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041430
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Signal recognition particle 54 kDa protein 
Protein Synonyms/Alias
  
Gene Name
 SRP54 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28KLVDPGVKAWTPTKGubiquitination[1]
85AGAFDQLKQNATKARubiquitination[2]
247IDKVNELKLDDNEALubiquitination[3, 4]
257DNEALIEKLKHGQFTubiquitination[4, 5]
259EALIEKLKHGQFTLRubiquitination[1]
334DGAKVFSKQPGRIQRubiquitination[1, 2]
362ELLTQYTKFAQMVKKubiquitination[1, 2, 4, 5]
374VKKMGGIKGLFKGGDubiquitination[1]
378GGIKGLFKGGDMSKNubiquitination[1, 2]
384FKGGDMSKNVSQSQMubiquitination[1, 2, 4]
393VSQSQMAKLNQQMAKubiquitination[1, 2, 4, 5, 6]
400KLNQQMAKMMDPRVLubiquitination[1, 2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 440 AA 
Protein Sequence
MASGLNKRKM IQHAVFKELV KLVDPGVKAW TPTKGKQNVI MFVGLQGSGK TTTCSKLAYY 60
YQRKGWKTCL ICADTFRAGA FDQLKQNATK ARIPFYGSYT EMDPVIIASE GVEKFKNENF 120
EIIIVDTSGR HKQEDSLFEE MLQVANAIQP DNIVYVMDAS IGQACEAQAK AFKDKVDVAS 180
VIVTKLDGHA KGGGALSAVA ATKSPIIFIG TGEHIDDFEP FKTQPFISKL LGMGDIEGLI 240
DKVNELKLDD NEALIEKLKH GQFTLRDMYE QFQNIMKMGP FSQILGMIPG FGTDFMSKGN 300
EQESMARLKK LMTIMDSMND QELDSTDGAK VFSKQPGRIQ RVARGSGVST RDVQELLTQY 360
TKFAQMVKKM GGIKGLFKGG DMSKNVSQSQ MAKLNQQMAK MMDPRVLHHM GGMAGLQSMM 420
RQFQQGAAGN MKGMMGFNNM 440 
Gene Ontology
 GO:0048500; C:signal recognition particle; IEA:InterPro.
 GO:0008312; F:7S RNA binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:InterPro.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR027417; P-loop_NTPase.
 IPR004125; Signal_recog_particle_SRP54_M.
 IPR006325; SRP54_euk.
 IPR000897; SRP54_GTPase_dom. 
Pfam
 PF00448; SRP54
 PF02978; SRP_SPB 
SMART
 SM00382; AAA
 SM00962; SRP54 
PROSITE
 PS00300; SRP54 
PRINTS