CPLM-006479

Genbank Nucleotide ID

Cyclin-dependent kinase 4

Protein Synonyms/Alias

Cell division protein kinase 4; PSK-J3

Rattus norvegicus (Rat)

Position	Peptide	Type	References
35	SGHFVALKSVRVPNG	acetylation	[1]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]

Functional Description

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

DOMAIN 6 295 Protein kinase.
NP_BIND 12 20 ATP (By similarity).
REGION 50 56 Required for binding D-type cyclins (By
ACT_SITE 140 140 Proton acceptor (By similarity).
BINDING 35 35 ATP (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 172 172 Phosphothreonine; by CAK (By similarity).

Acetylation; ATP-binding; Cell cycle; Cell division; Complete proteome; Cytoplasm; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Serine/threonine-protein kinase; Transferase.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0000785; C:chromatin; IEA:Compara.
GO:0000307; C:cyclin-dependent protein kinase holoenzyme complex; IEA:Compara.
GO:0005829; C:cytosol; IEA:Compara.
GO:0031965; C:nuclear membrane; IEA:Compara.
GO:0005730; C:nucleolus; IEA:Compara.
GO:0005634; C:nucleus; IDA:RGD.
GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
GO:0005923; C:tight junction; IEA:Compara.
GO:0005667; C:transcription factor complex; IEA:Compara.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IDA:RGD.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0007623; P:circadian rhythm; IEP:RGD.
GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
GO:0031100; P:organ regeneration; IEP:RGD.
GO:0043065; P:positive regulation of apoptotic process; IMP:RGD.
GO:0045787; P:positive regulation of cell cycle; TAS:RGD.
GO:0008284; P:positive regulation of cell proliferation; IMP:RGD.
GO:0045793; P:positive regulation of cell size; IMP:RGD.
GO:0048146; P:positive regulation of fibroblast proliferation; IEA:Compara.
GO:0045727; P:positive regulation of translation; IMP:RGD.
GO:0042493; P:response to drug; IEA:Compara.
GO:0055093; P:response to hyperoxia; IEP:RGD.
GO:0010288; P:response to lead ion; IMP:RGD.
GO:0033574; P:response to testosterone stimulus; IDA:RGD.
GO:0009636; P:response to toxic substance; IEP:RGD.
GO:0007165; P:signal transduction; IEA:Compara.

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST