CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021983
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Synembryn-A 
Protein Synonyms/Alias
 Protein Ric-8A 
Gene Name
 RIC8A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
328LHKTHRLKESVAPVLubiquitination[1]
353RPARKFLKAQVLPPLubiquitination[1, 2]
409ESVPRFIKYTGYGNAubiquitination[2]
489KLVTMFDKLSRNRVIubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation. 
Sequence Annotation
 MOD_RES 199 199 Phosphothreonine (By similarity).
 MOD_RES 436 436 Phosphoserine.
 MOD_RES 441 441 Phosphothreonine.
 MOD_RES 502 502 Phosphoserine.
 MOD_RES 523 523 Phosphoserine.
 MOD_RES 524 524 Phosphoserine.
 MOD_RES 528 528 Phosphoserine.  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MEPRAVAEAV ETGEEDVIME ALRSYNQEHS QSFTFDDAQQ EDRKRLAELL VSVLEQGLPP 60
SHRVIWLQSV RILSRDRNCL DPFTSRQSLQ ALACYADISV SEGSVPESAD MDVVLESLKC 120
LCNLVLSSPV AQMLAAEARL VVKLTERVGL YRERSFPHDV QFFDLRLLFL LTALRTDVRQ 180
QLFQELKGVR LLTDTLELTL GVTPEGNPPP TLLPSQETER AMEILKVLFN ITLDSIKGEV 240
DEEDAALYRH LGTLLRHCVM IATAGDRTEE FHGHAVNLLG NLPLKCLDVL LTLEPHGDST 300
EFMGVNMDVI RALLIFLEKR LHKTHRLKES VAPVLSVLTE CARMHRPARK FLKAQVLPPL 360
RDVRTRPEVG EMLRNKLVRL MTHLDTDVKR VAAEFLFVLC SESVPRFIKY TGYGNAAGLL 420
AARGLMAGGR PEGQYSEDED TDTDEYKEAK ASINPVTGRV EEKPPNPMEG MTEEQKEHEA 480
MKLVTMFDKL SRNRVIQPMG MSPRGHLTSL QDAMCETMEQ QLSSDPDSDP D 531 
Gene Ontology
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
 GO:0007193; P:adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway; IEA:Compara.
 GO:0071711; P:basement membrane organization; IEA:Compara.
 GO:0042074; P:cell migration involved in gastrulation; IEA:Compara.
 GO:0070586; P:cell-cell adhesion involved in gastrulation; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0001944; P:vasculature development; IEA:Compara.
 GO:0008542; P:visual learning; IEA:Compara. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR019318; Gua_nucleotide_exch_fac_Ric8.
 IPR008376; Synembryn. 
Pfam
 PF10165; Ric8 
SMART
  
PROSITE
  
PRINTS
 PR01802; SYNEMBRYN.