UniProt Accession

 UBS3A_HUMAN; P57075; G5E9E4; Q6HA34; Q6HA35; Q6ISI6; Q6ISK3; Q6ISS9 

Genbank Protein ID

 AJ277750; AF520809; AF521702; AP001746; CH471079; BC069357; BC069483; BC069511; BC069577 

Genbank Nucleotide ID

 CAB91543.1; AAP80731.1; AAP80738.1; EAX09560.1; AAH69357.1; AAH69483.1; AAH69511.1 

Protein Name

 Ubiquitin-associated and SH3 domain-containing protein A 

Protein Synonyms/Alias

 Cbl-interacting protein 4; CLIP4; Suppressor of T-cell receptor signaling 2; STS-2; T-cell ubiquitin ligand 1; TULA-1 

Gene Name

 UBASH3A 

Gene Synonyms/Alias

 STS2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
11	GETQLYAKVSNKLKS	ubiquitination	[1, 2]
240	LHLTLAHKFYPHHQR	ubiquitination	[2, 3]
347	SESDTWVKHRMYTFS	ubiquitination	[2, 3]
396	LQATVARKSVLVVRH	ubiquitination	[2]
413	RVDQIFGKAWLQQCS	ubiquitination	[1, 2, 3]
425	QCSTPDGKYYRPDLN	ubiquitination	[1, 2, 3]
445	PRRSRGIKDFENDPP	ubiquitination	[1, 2]
498	KLILEELKLEKKIKI	ubiquitination	[2, 3]
501	LEELKLEKKIKIRVE	ubiquitination	[2, 3]
516	PGIFEWTKWEAGKTT	ubiquitination	[2, 3]
521	WTKWEAGKTTPTLMS	ubiquitination	[2, 3]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain. 

Sequence Annotation

 DOMAIN 15 60 UBA.
 DOMAIN 276 340 SH3.
 REGION 395 661 Phosphatase-like.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Polymorphism; Reference proteome; SH3 domain. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 661 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0050860; P:negative regulation of T cell receptor signaling pathway; IEA:Compara.
 GO:0001817; P:regulation of cytokine production; IEA:Compara.
 GO:0016070; P:RNA metabolic process; IEA:InterPro. 

Interpro

 IPR013078; His_Pase_superF_clade-1.
 IPR009097; RNA_ligase/cNuc_Pdiesterase.
 IPR001452; SH3_domain.
 IPR009060; UBA-like.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 

Pfam

 PF00300; His_Phos_1
 PF00018; SH3_1 

SMART

 SM00326; SH3 

PROSITE

 PS50002; SH3
 PS50030; UBA 

PRINTS