UniProt Accession

 IF5A1_YEAST; P23301; D3DLL5 

Genbank Protein ID

 M63541; X56236; D83166; U18779; BK006939 

Genbank Nucleotide ID

 AAA35155.1; CAA39693.1; BAA11826.1; AAB65008.1; DAA07619.1 

Protein Name

 Eukaryotic translation initiation factor 5A-1 

Protein Synonyms/Alias

 eIF-5A-1; Hypusine-containing protein HP2; eIF-4D 

Gene Name

 HYP2 

Gene Synonyms/Alias

 TIF51A; YEL034W; SYGP-ORF21 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
35	KNGFVVIKSRPCKIV	acetylation	[1]
35	KNGFVVIKSRPCKIV	ubiquitination	[2]
40	VIKSRPCKIVDMSTS	acetylation	[1]
40	VIKSRPCKIVDMSTS	ubiquitination	[2]
68	AIDIFTGKKLEDLSP	ubiquitination	[3]
69	IDIFTGKKLEDLSPS	acetylation	[1, 4]
86	NMEVPVVKRNEYQLL	acetylation	[1]
86	NMEVPVVKRNEYQLL	ubiquitination	[3]
114	GDTKDDVKAPEGELG	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Preparative peptide isoelectric focusing as a tool for improving the identification of lysine-acetylated peptides from complex mixtures.
 Xie H, Bandhakavi S, Roe MR, Griffin TJ.
 J Proteome Res. 2007 May;6(5):2019-26. [PMID: 17397211] 

Functional Description

 mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Essential for polarized growth, a process necessary for G1/S transition. May mediate large range of effects of the polyamine spermidine in the cell. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 7 7 Phosphothreonine (By similarity).
 MOD_RES 10 10 Phosphothreonine.
 MOD_RES 51 51 Hypusine.
 MOD_RES 74 74 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Elongation factor; Hypusine; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 157 AA 

Protein Sequence

Gene Ontology

 GO:0022626; C:cytosolic ribosome; IPI:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0043022; F:ribosome binding; IPI:SGD.
 GO:0003746; F:translation elongation factor activity; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0008612; P:peptidyl-lysine modification to hypusine; IEA:InterPro.
 GO:0045901; P:positive regulation of translational elongation; IDA:SGD.
 GO:0045948; P:positive regulation of translational initiation; IDA:SGD.
 GO:0045905; P:positive regulation of translational termination; IDA:SGD.
 GO:0006452; P:translational frameshifting; IMP:SGD. 

Interpro

 IPR005824; KOW.
 IPR012340; NA-bd_OB-fold.
 IPR014722; Rib_L2_dom2.
 IPR019769; Trans_elong_IF5A_hypusine_site.
 IPR001884; Transl_elong_IF5A.
 IPR020189; Transl_elong_IF5A_C.
 IPR008991; Translation_prot_SH3-like. 

Pfam

 PF01287; eIF-5a
 PF00467; KOW 

SMART

  

PROSITE

 PS00302; IF5A_HYPUSINE 

PRINTS