UniProt Accession

 RAP1_YEAST; P11938; D6W0X4 

Genbank Protein ID

 M18068; X78898; Z71492; BK006947 

Genbank Nucleotide ID

 AAA18404.1; CAA55491.1; CAA96118.1; DAA10340.1 

Protein Name

 DNA-binding protein RAP1 

Protein Synonyms/Alias

 Repressor/activator site-binding protein; SBF-E; TUF 

Gene Name

 RAP1 

Gene Synonyms/Alias

 GRF1; TUF1; YNL216W; N1310 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
164	GGEVLDSKPRESKEN	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Essential regulatory protein in yeast whose DNA-binding sites are found at three types of chromosomal elements: promoters, silencers, and telomeres. RAP1 is also involved in the regulation of telomere structure, where its binding sites are found within the terminal poly[C(1-3)A] sequences. The opposite regulatory functions of RAP1 are not intrinsic to its binding sites but, instead, result from interactions with different factors at promoters and silencers. RAP1 associates with SIR3 and SIR4 proteins to form a DNA-binding complex that initiates the repression at the HM loci and telomeres. May also target the binding of RIF1 and RIF2 to silencers and telomeres. Forms with GCR1 a transcriptional activation complex that is required for expression of glycolytic and ribosomal gene. 

Sequence Annotation

 DOMAIN 121 208 BRCT.
 DOMAIN 355 415 HTH myb-type.
 DNA_BIND 388 411 H-T-H motif.
 REGION 630 695 Activation domain.
 MOD_RES 486 486 Phosphothreonine.
 MOD_RES 731 731 Phosphoserine.  

Keyword

 3D-structure; Activator; Chromosome; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Telomere; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 827 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0000788; C:nuclear nucleosome; IDA:SGD.
 GO:0000783; C:nuclear telomere cap complex; IDA:SGD.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IDA:SGD.
 GO:0008301; F:DNA binding, bending; IDA:SGD.
 GO:0051880; F:G-quadruplex DNA binding; IDA:SGD.
 GO:0031492; F:nucleosomal DNA binding; IDA:SGD.
 GO:0000982; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; IDA:SGD.
 GO:0001132; F:TBP-class protein binding RNA polymerase II transcription factor activity; IDA:SGD.
 GO:0042162; F:telomeric DNA binding; IDA:SGD.
 GO:0001094; F:TFIID-class transcription factor binding; IDA:SGD.
 GO:0030466; P:chromatin silencing at silent mating-type cassette; IGI:SGD.
 GO:0035390; P:establishment of chromatin silencing at telomere; IPI:SGD.
 GO:0071169; P:establishment of protein localization to chromatin; IPI:SGD.
 GO:0070200; P:establishment of protein localization to telomere; IPI:SGD.
 GO:0031936; P:negative regulation of chromatin silencing; IDA:SGD.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:0031848; P:protection from non-homologous end joining at telomere; IMP:SGD.
 GO:0072363; P:regulation of glycolysis by positive regulation of transcription from RNA polymerase II promoter; IGI:SGD.
 GO:0034401; P:regulation of transcription by chromatin organization; IDA:SGD.
 GO:0010833; P:telomere maintenance via telomere lengthening; IMP:SGD. 

Interpro

 IPR001357; BRCT_dom.
 IPR009057; Homeodomain-like.
 IPR017930; Myb_dom.
 IPR021661; Rap1_C.
 IPR015280; Rap1_DNA-bd.
 IPR001005; SANT/Myb. 

Pfam

 PF00249; Myb_DNA-binding
 PF09197; Rap1-DNA-bind
 PF11626; Rap1_C 

SMART

 SM00292; BRCT
 SM00717; SANT 

PROSITE

 PS50172; BRCT
 PS51294; HTH_MYB 

PRINTS