CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023044
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Drebrin-like protein 
Protein Synonyms/Alias
 Cervical SH3P7; Cervical mucin-associated protein; Drebrin-F; HPK1-interacting protein of 55 kDa; HIP-55; SH3 domain-containing protein 7 
Gene Name
 DBNL 
Gene Synonyms/Alias
 CMAP; SH3P7; PP5423 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
23YVRVVTEKSPTDWALubiquitination[1]
134CIMEKVAKASGANYSubiquitination[1]
144GANYSFHKESGRFQDubiquitination[1, 2]
164PVGSVYQKTNAVSEIubiquitination[1, 3]
176SEIKRVGKDSFWAKAubiquitination[1]
289ISSPQPGKLRSPFLQubiquitination[1]
297LRSPFLQKQLTQPETubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G- actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes (By similarity). May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. 
Sequence Annotation
 DOMAIN 4 133 ADF-H.
 DOMAIN 371 430 SH3.
 MOD_RES 176 176 N6-acetyllysine.
 MOD_RES 232 232 Phosphoserine.
 MOD_RES 269 269 Phosphoserine.
 MOD_RES 270 270 Phosphothreonine (By similarity).
 MOD_RES 283 283 Phosphoserine.
 MOD_RES 288 288 N6-acetyllysine.
 MOD_RES 291 291 Phosphothreonine (By similarity).
 MOD_RES 334 334 Phosphotyrosine (By similarity).
 MOD_RES 344 344 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Acetylation; Actin-binding; Adaptive immunity; Alternative splicing; Cell junction; Cell membrane; Cell projection; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Endocytosis; Golgi apparatus; Immunity; Membrane; Phosphoprotein; Reference proteome; SH3 domain; Synapse; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 430 AA 
Protein Sequence
MAANLSRNGP ALQEAYVRVV TEKSPTDWAL FTYEGNSNDI RVAGTGEGGL EEMVEELNSG 60
KVMYAFCRVK DPNSGLPKFV LINWTGEGVN DVRKGACASH VSTMASFLKG AHVTINARAE 120
EDVEPECIME KVAKASGANY SFHKESGRFQ DVGPQAPVGS VYQKTNAVSE IKRVGKDSFW 180
AKAEKEEENR RLEEKRRAEE AQRQLEQERR ERELREAARR EQRYQEQGGE ASPQSRTWEQ 240
QQEVVSRNRN EQGSTCASLQ ESAVHPREIF KQKERAMSTT SISSPQPGKL RSPFLQKQLT 300
QPETHFGREP AAAISRPRAD LPAEEPAPST PPCLVQAEEE AVYEEPPEQE TFYEQPPLVQ 360
QQGAGSEHID HHIQGQGLSG QGLCARALYD YQAADDTEIS FDPENLITGI EVIDEGWWRG 420
YGPDGHFGMF PANYVELIE 439 
Gene Ontology
 GO:0005938; C:cell cortex; ISS:UniProtKB.
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0030027; C:lamellipodium; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0002102; C:podosome; ISS:UniProtKB.
 GO:0014069; C:postsynaptic density; IEA:Compara.
 GO:0001726; C:ruffle; ISS:UniProtKB.
 GO:0051015; F:actin filament binding; ISS:UniProtKB.
 GO:0008047; F:enzyme activator activity; TAS:ProtInc.
 GO:0007257; P:activation of JUN kinase activity; TAS:ProtInc.
 GO:0006921; P:cellular component disassembly involved in execution phase of apoptosis; TAS:Reactome.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0048812; P:neuron projection morphogenesis; ISS:UniProtKB.
 GO:0071800; P:podosome assembly; ISS:UniProtKB.
 GO:0016601; P:Rac protein signal transduction; ISS:UniProtKB.
 GO:0007416; P:synapse assembly; ISS:UniProtKB. 
Interpro
 IPR002108; Actin-bd_cofilin/tropomyosin.
 IPR001452; SH3_domain. 
Pfam
 PF00241; Cofilin_ADF
 PF00018; SH3_1 
SMART
 SM00102; ADF
 SM00326; SH3 
PROSITE
 PS51263; ADF_H
 PS50002; SH3 
PRINTS