UniProt Accession

 NT5C_HUMAN; Q8TCD5; Q96HS6; Q9NP82 

Genbank Protein ID

 AF154829; AK000419; AC022211; BC008183; BC017454; BC022334; BK000192 

Genbank Nucleotide ID

 AAF36534.2; BAA91151.1; AAH08183.1; AAH17454.1; AAH22334.1; DAA00070.1 

Protein Name

 5'(3')-deoxyribonucleotidase, cytosolic type 

Protein Synonyms/Alias

 Cytosolic 5',3'-pyrimidine nucleotidase; Deoxy-5'-nucleotidase 1; dNT-1 

Gene Name

 NT5C 

Gene Synonyms/Alias

 DNT1; UMPH2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
146	GDLLIDDKDTVRGQE	ubiquitination	[1]
194	WREILDSKRGAAQRE	ubiquitination	[1, 2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP. 

Sequence Annotation

 ACT_SITE 10 10 Nucleophile (Probable).
 ACT_SITE 12 12 Proton donor (Probable).
 METAL 10 10 Magnesium.
 METAL 12 12 Magnesium; via carbonyl oxygen.
 METAL 145 145 Magnesium.
 BINDING 18 18 Substrate.
 BINDING 44 44 Substrate.
 BINDING 65 65 Substrate (By similarity).
 BINDING 99 99 Substrate (Probable).
 BINDING 134 134 Substrate (Probable).
 MOD_RES 182 182 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Magnesium; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 201 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0008253; F:5'-nucleotidase activity; EXP:Reactome.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0019103; F:pyrimidine nucleotide binding; IDA:UniProtKB.
 GO:0006144; P:purine nucleobase metabolic process; TAS:Reactome.
 GO:0006195; P:purine nucleotide catabolic process; TAS:Reactome.
 GO:0009223; P:pyrimidine deoxyribonucleotide catabolic process; IDA:UniProtKB.
 GO:0006206; P:pyrimidine nucleobase metabolic process; TAS:Reactome.
 GO:0046135; P:pyrimidine nucleoside catabolic process; TAS:Reactome. 

Interpro

 IPR010708; 5'(3')-deoxyribonucleotidase.
 IPR023214; HAD-like_dom. 

Pfam

 PF06941; NT5C 

SMART

  

PROSITE

  

PRINTS