CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021928
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 6 
Protein Synonyms/Alias
  
Gene Name
 WDR6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
237DRSVRIWKVGDLRVPubiquitination[1, 2, 3]
343GVSALCFKSRSRPGTubiquitination[2, 4]
352RSRPGTLKAVTLAGSubiquitination[2]
418ANGEGRVKVVPINTPubiquitination[2, 4, 5, 6]
437DQTLFPGKVHSLSWAubiquitination[1, 2, 3, 4]
490RYLLPPSKQRWHTCSubiquitination[1, 2, 3, 4, 5]
526PSRPGLLKDPGVGGKubiquitination[1, 2, 3, 4, 5, 6]
647WNPRSHEKLHIVNCGubiquitination[2]
708GREITCVKRVGTITLubiquitination[2]
855RNRHRMVKVDPETRYubiquitination[2, 5, 6]
910LAETFHHKRCVLKVHubiquitination[2, 4, 6]
915HHKRCVLKVHSFTHEubiquitination[2, 5]
1052AAHVTGLKILSPSIMubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Enhances the STK11/LKB1-induced cell growth suppression activity. Negative regulator of amino acid starvation-induced autophagy. 
Sequence Annotation
 REPEAT 89 130 WD 1.
 REPEAT 155 197 WD 2.
 REPEAT 207 246 WD 3.
 REPEAT 256 294 WD 4.
 REPEAT 295 335 WD 5.
 REPEAT 346 384 WD 6.
 REPEAT 433 475 WD 7.
 REPEAT 489 528 WD 8.
 REPEAT 567 605 WD 9.
 REPEAT 611 650 WD 10.
 REPEAT 652 692 WD 11.
 REPEAT 747 793 WD 12.
 REPEAT 860 901 WD 13.
 REPEAT 908 954 WD 14.
 REPEAT 978 1020 WD 15.
 REPEAT 1043 1081 WD 16.  
Keyword
 Cell cycle; Complete proteome; Cytoplasm; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1121 AA 
Protein Sequence
MDALEDYVWP RATSELILLP VTGLECVGDR LLAGEGPDVL VYSLDFGGHL RMIKRVQNLL 60
GHYLIHGFRV RPEPNGDLDL EAMVAVFGSK GLRVVKISWG QGHFWELWRS GLWNMSDWIW 120
DARWLEGNIA LALGHNSVVL YDPVVGCILQ EVPCTDRCTL SSACLIGDAW KELTIVAGAV 180
SNQLLVWYPA TALADNKPVA PDRRISGHVG IIFSMSYLES KGLLATASED RSVRIWKVGD 240
LRVPGGRVQN IGHCFGHSAR VWQVKLLENY LISAGEDCVC LVWSHEGEIL QAFRGHQGRG 300
IRAIAAHERQ AWVITGGDDS GIRLWHLVGR GYRGLGVSAL CFKSRSRPGT LKAVTLAGSW 360
RLLAVTDTGA LYLYDVEVKC WEQLLEDKHF QSYCLLEAAP GPEGFGLCAM ANGEGRVKVV 420
PINTPTAAVD QTLFPGKVHS LSWALRGYEE LLLLASGPGG VVACLEISAA PSGKAIFVKE 480
RCRYLLPPSK QRWHTCSAFL PPGDFLVCGD RRGSVLLFPS RPGLLKDPGV GGKARAGAGA 540
PVVGSGSSGG GNAFTGLGPV STLPSLHGKQ GVTSVTCHGG YVYTTGRDGA YYQLFVRDGQ 600
LQPVLRQKSC RGMNWLAGLR IVPDGSMVIL GFHANEFVVW NPRSHEKLHI VNCGGGHRSW 660
AFSDTEAAMA FAYLKDGDVM LYRALGGCTR PHVILREGLH GREITCVKRV GTITLGPEYG 720
VPSFMQPDDL EPGSEGPDLT DIVITCSEDT TVCVLALPTT TGSAHALTAV CNHISSVRAV 780
AVWGIGTPGG PQDPQPGLTA HVVSAGGRAE MHCFSIMVTP DPSTPSRLAC HVMHLSSHRL 840
DEYWDRQRNR HRMVKVDPET RYMSLAVCEL DQPGLGPLVA AACSDGAVRL FLLQDSGRIL 900
QLLAETFHHK RCVLKVHSFT HEAPNQRRRL LLCSAATDGS LAFWDLTTML DHDSTVLEPP 960
VDPGLPYRLG TPSLTLQAHS CGINSLHTLP TREGHHLVAS GSEDGSLHVF VLAVEMLQLE 1020
EAVGEAGLVP QLRVLEEYSV PCAHAAHVTG LKILSPSIMV SASIDQRLTF WRLGHGEPTF 1080
MNSTVFHVPD VADMDCWPVS PEFGHRCALG GQGLEVYNWY D 1121 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0007050; P:cell cycle arrest; IDA:UniProtKB.
 GO:0010507; P:negative regulation of autophagy; IMP:BHF-UCL.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB. 
Interpro
 IPR011044; Quino_amine_DH_bsu.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS