UniProt Accession

 RTF1_YEAST; P53064; D6VV91; P89115 

Genbank Protein ID

 U86702; Z49149; Z72766; Z72767; BK006941 

Genbank Nucleotide ID

 AAB47535.1; CAA89011.1; CAA96963.1; CAA96965.1; DAA07875.1 

Protein Name

 RNA polymerase-associated protein RTF1 

Protein Synonyms/Alias

  

Gene Name

 RTF1 

Gene Synonyms/Alias

 CSL3; YGL244W; HRA458 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
116	ERSQIMQKYQERKLF	acetylation	[1]
165	HSDIKASKLSQLKKQ	acetylation	[1]
280	RVNVGTDKRTGKTSY	acetylation	[1]
412	REKLQYAKETNNEKD	acetylation	[1]
422	NNEKDIAKYSAQLRN	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 The PAF1 complex is a multifunctional complex. Involved in transcription initiation via genetic interactions with TATA- binding proteins. Involved in elongation. It regulates 3'-end formation of snR47 by modulating the recruitment or stable association of NRD1 and NAB3 with RNA polymerase II. Also has a role in transcription-coupled histone modification. Required for activation of RAD6 ubiquitin conjugate and the BRE1 ubiquitin ligase which ubiquitinate 'Lys-126' histone H2B. Activates the SET1 histone methyltransferase complex for methylation of 'Lys-4' of histone H3 and for methylation of 'Lys-73' of histone H3 by DOT1 and 'Lys-36' of histone H3 by SET2. Important for TATA site selection by TBP. Directly or indirectly regulates the DNA-binding properties of SPT15, the TATA box-binding protein, and the relative activities of different TATA elements. 

Sequence Annotation

 DOMAIN 238 370 Plus3.
 MOD_RES 17 17 Phosphoserine.
 MOD_RES 69 69 Phosphotyrosine.
 MOD_RES 71 71 Phosphoserine.
 MOD_RES 477 477 Phosphoserine.  

Keyword

 Activator; Complete proteome; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 558 AA 

Protein Sequence

Gene Ontology

 GO:0016593; C:Cdc73/Paf1 complex; IPI:SGD.
 GO:0035327; C:transcriptionally active chromatin; IDA:SGD.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003723; F:RNA binding; IDA:SGD.
 GO:0001076; F:RNA polymerase II transcription factor binding transcription factor activity; IPI:SGD.
 GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
 GO:0070911; P:global genome nucleotide-excision repair; IMP:SGD.
 GO:0016570; P:histone modification; IEA:InterPro.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:SGD.
 GO:2001165; P:positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues; IMP:SGD.
 GO:2001209; P:positive regulation of transcription elongation from RNA polymerase I promoter; IDA:SGD.
 GO:0032968; P:positive regulation of transcription elongation from RNA polymerase II promoter; IMP:SGD.
 GO:0034402; P:recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex; IMP:SGD.
 GO:0031938; P:regulation of chromatin silencing at telomere; IMP:SGD.
 GO:2001173; P:regulation of histone H2B conserved C-terminal lysine ubiquitination; IDA:SGD.
 GO:0051569; P:regulation of histone H3-K4 methylation; IMP:SGD.
 GO:2001160; P:regulation of histone H3-K79 methylation; IMP:SGD.
 GO:0090262; P:regulation of transcription-coupled nucleotide-excision repair; IGI:SGD.
 GO:0031126; P:snoRNA 3'-end processing; IMP:SGD.
 GO:0001015; P:snoRNA transcription from an RNA polymerase II promoter; IMP:SGD.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; IMP:SGD. 

Interpro

 IPR004343; Plus-3.
 IPR018144; Plus3-dom_subgr. 

Pfam

 PF03126; Plus-3 

SMART

 SM00719; Plus3 

PROSITE

 PS51360; PLUS3 

PRINTS