CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014118
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription initiation factor TFIID subunit 3 
Protein Synonyms/Alias
 140 kDa TATA box-binding protein-associated factor; TBP-associated factor 3; Transcription initiation factor TFIID 140 kDa subunit; TAF(II)140; TAF140; TAFII-140; TAFII140 
Gene Name
 TAF3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
261KSQMPTAKPLETKSFacetylation[1]
266TAKPLETKSFTPKTKacetylation[2, 3, 4]
776QDKIVISKVVPAPEAacetylation[2, 3]
784VVPAPEAKPAPSQNRacetylation[3]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process. 
Sequence Annotation
 ZN_FING 865 915 PHD-type.
 MOD_RES 183 183 Phosphoserine.
 MOD_RES 229 229 Phosphoserine.
 MOD_RES 243 243 Phosphoserine.
 MOD_RES 266 266 N6-acetyllysine.
 MOD_RES 297 297 Phosphoserine.
 MOD_RES 301 301 Phosphoserine.
 MOD_RES 501 501 Phosphothreonine.
 MOD_RES 776 776 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 929 AA 
Protein Sequence
MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH RYSELYGRTD 60
PILDDVGEAF QLMGVSLHEL EDYIHNIEPV TFPHQIPSFP VSKNNVLQFP QPGSKDAEER 120
KEYIPDYLPP IVSSQEEEEE EQVPTDGGTS AEAMQVPLEE DDELEEEEII NDENFLGKRP 180
LDSPEAEELP AMKRPRLLST KGDTLDVVLL EAREPLSSIN TQKIPPMLSP VHVQDSTDLA 240
PPSPEPPMLA PVAKSQMPTA KPLETKSFTP KTKTKTSSPG QKTKSPKTAQ SPAMVGSPIR 300
SPKTVSKEKK SPGRSKSPKS PKSPKVTTHI PQTPVRPETP NRTPSATLSE KISKETIQVK 360
QIQTPPDAGK LNSENQPKKA VVADKTIEAS IDAVIARACA EREPDPFEFS SGSESEGDIF 420
TSPKRISGPE CTTPKASTSA NNFTKSGSTP LPLSGGTSSS DNSWTMDASI DEVVRKAKLG 480
TPSNMPPNFP YISSPSVSPP TPEPLHKVYE EKTKLPSSVE VKKKLKKELK TKMKKKEKQR 540
DREREKDKNK DKSKEKDKVK EKEKDKETGR ETKYPWKEFL KEEEADPYKF KIKEFEDVDP 600
KVKLKDGLVR KEKEKHKDKK KDREKGKKDK DKREKEKVKD KGREDKMKAP APPLVLPPKE 660
LALPLFSPAT ASRVPAMLPS LLPVLPEKLF EEKEKVKEKE KKKDKKEKKK KKEKEKEKKE 720
KEREKEKRER EKREKEKEKH KHEKIKVEPV ALAPSPVIPR LTLRVGAGQD KIVISKVVPA 780
PEAKPAPSQN RPKTPPPAPA PAPGPMLVSP APVPLPLLAQ AAAGPALLPS PGPAASGASA 840
KAPVRSVVTE TVSTYVIRDE WGNQIWICPG CNKPDDGSPM IGCDDCDDWY HWPCVGIMTA 900
PPEEMQWFCP KCANKKKDKK HKKRKHRAH 929 
Gene Ontology
 GO:0005669; C:transcription factor TFIID complex; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0051457; P:maintenance of protein location in nucleus; IDA:HGNC.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR006565; BTP.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR019787; Znf_PHD-finger.
 IPR013083; Znf_RING/FYVE/PHD. 
Pfam
 PF07524; Bromo_TP
 PF00628; PHD 
SMART
 SM00576; BTP
 SM00249; PHD 
PROSITE
 PS01359; ZF_PHD_1
 PS50016; ZF_PHD_2 
PRINTS