CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015946
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Golgin subfamily A member 7 
Protein Synonyms/Alias
 Golgi complex-associated protein of 16 kDa 
Gene Name
 GOLGA7 
Gene Synonyms/Alias
 GCP16; HDCKB03P; HSPC041 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
11QQAPVSGKVFIQRDYubiquitination[1]
29TRCQFQTKFPAELENubiquitination[1, 2, 3, 4, 5]
92HYEKVLKKVSKYIQEubiquitination[6]
95KVLKKVSKYIQEQNEubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9]
103YIQEQNEKIYAPQGLubiquitination[1, 3, 4, 6, 8, 9, 10]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
 May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. 
Sequence Annotation
 LIPID 69 69 S-palmitoyl cysteine.
 LIPID 72 72 S-palmitoyl cysteine.  
Keyword
 Alternative splicing; Complete proteome; Golgi apparatus; Lipoprotein; Membrane; Palmitate; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 137 AA 
Protein Sequence
MRPQQAPVSG KVFIQRDYSS GTRCQFQTKF PAELENRIDR QQFEETVRTL NNLYAEAEKL 60
GGQSYLEGCL ACLTAYTIFL CMETHYEKVL KKVSKYIQEQ NEKIYAPQGL LLTDPIERGL 120
RVIEITIYED RGMSSGR 137 
Gene Ontology
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell. 
Interpro
 IPR019383; Golgin_A_7/ERF4. 
Pfam
 PF10256; Erf4 
SMART
  
PROSITE
  
PRINTS