CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007921
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bud site selection protein 4 
Protein Synonyms/Alias
  
Gene Name
 BUD4 
Gene Synonyms/Alias
 YJR092W; J1905 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
346DDAFDEKKVLDEGCSubiquitination[1]
373RSIVYSNKGTNANVQubiquitination[1]
431ESYIADYKVTRQEDWubiquitination[1]
441RQEDWDTKKLHQESEubiquitination[1]
481SEFQRNFKDGEEYRIubiquitination[1]
691RSRIYNPKSRVSSLNubiquitination[1]
726RRNTLISKRIQDNIRubiquitination[1]
866FQRDSSKKSFNSLWEubiquitination[1]
879WESSYELKPPPSIRKubiquitination[1]
886KPPPSIRKQPIAPDVubiquitination[1]
903KLLESDTKDDADLEKubiquitination[1]
992VRSISPIKRHVSSPFubiquitination[1]
1000RHVSSPFKVVKAGNKubiquitination[1]
1007KVVKAGNKQENNEINubiquitination[1]
1033QQETDGLKQDIPPLLubiquitination[1]
1123DGNIRINKEFELPIDubiquitination[1]
1285SAFEQFPKQFSLVNKubiquitination[1]
1401AECSNEEKSDWYNKLubiquitination[1]
1434EKLAEEEKTRTTGHNubiquitination[1]
1443RTTGHNLKQDFN***ubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for establishment of the axial budding pattern in haploid cells. Cooperates with other bud site selection proteins to recognize a spatial landmark during mitosis and they subsequently become a landmark for downstream polarity establishment factors that coordinate axial budding and cytokinesis. Involved in the septin organization at the bud neck. 
Sequence Annotation
 DOMAIN 1302 1413 PH.
 REGION 768 879 Interaction with IQG1.
 MOD_RES 10 10 Phosphoserine.
 MOD_RES 78 78 Phosphoserine.
 MOD_RES 81 81 Phosphoserine.
 MOD_RES 91 91 Phosphoserine.
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 167 167 Phosphoserine.
 MOD_RES 365 365 Phosphothreonine.
 MOD_RES 367 367 Phosphoserine.
 MOD_RES 511 511 Phosphoserine.
 MOD_RES 616 616 Phosphoserine.
 MOD_RES 805 805 Phosphoserine.
 MOD_RES 811 811 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Complete proteome; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1447 AA 
Protein Sequence
MHDAESTVDS LLKEIDNEME QTKSNITQNG SEDTPHNWKL PLQEIGDDTM EMLVKHNTRS 60
NATENSRGRS PSKMSTISNE SLNLGLLRVN SELEESPAAV HQERIKNSVA NGALGHANSP 120
KVLNNLKNMA QDIDKLARDE EKPVKLSSSP LKFTLKSTQP LLSYPESPIH RSSIEIETNY 180
DDEDEEEEDA YTCLTQSPQI LHSPSRIPIT NAVSINKLNL DFTLNPNESD KSLVSDTSVD 240
STGRELDTKT IPELPFCMSS TPEMTPVDEK CNLPSKLLNT SNNSHSDSRS PTASVEDLNI 300
STNLPGADSS QNNPVTTDAD ALIENDVVRD LQQNMEHIDD AFDEKKVLDE GCSNEPVTFL 360
GENDTRSIVY SNKGTNANVQ EFSQEDSLAH SEPKFKDLNA TSDDVWNEDK ETDANISTST 420
KSEESYIADY KVTRQEDWDT KKLHQESEHA NEQPAIIPQK DSSEETFTEL NNESEFQRNF 480
KDGEEYRIVQ HEESLYGQRT KSPEENIING SEIGVDHGEA AEVNEPLAKT SAEEHDLSSS 540
CEDQSVSEAR NKDRIEEKEV ETKDENIETE KDESEYHKVE ENEEPEHVPL LPPLPRWEEI 600
QFNEPFIDEN DTSNDSIDLT RSMKPSDYIS IWHIQEEEIK SNSPESIANS QFSQQSSITT 660
ASTVDSKKDN GSTSFKFKPR IVSRSRIYNP KSRVSSLNYY DNEDYILSNS EWNALDPMRR 720
NTLISKRIQD NIRTQKGHAP LIRPSIMKLN GEDSGFQNHF LEVEQPQEHE NIPLSTHLSE 780
QDITTNVGLD EQKLPTNTQD EAEISIREIE SAGDITFNRG DLLSLSFDEE LGQDFANFLD 840
ALDHDSTSFN HGPDDSSSFQ RDSSKKSFNS LWESSYELKP PPSIRKQPIA PDVLQKLLES 900
DTKDDADLEK IREERITEPR TGLGIGMLKT PVKDVSIALA ASIKGYEASF SDTDSRPEGM 960
NNSDAITLNM FDDFEEDKMT PSTPVRSISP IKRHVSSPFK VVKAGNKQEN NEINIKAEEE 1020
IEPMTQQETD GLKQDIPPLL AQTKDNVEAK EETITQLEEP QDVEQEFPDM GTLYLSIKAI 1080
STLALYGTKS HRATYAIVFD NGENVVQTPW ESLPYDGNIR INKEFELPID FKGKAETSSA 1140
SSERDSYKKC VITLKCKYEK PRHELVEIVD KVPVGKSFFG KTKYKFEKKY VQKKPKQDEW 1200
DYLFAQDGSF ARCEIEINEE FLKNVAFNTS HMHYNMINKW SRIADKIHGS KRLYELPRKA 1260
PHKVASLDVE ACFLERTSAF EQFPKQFSLV NKIVSKYKLQ QNIYKEGYLL QDGGDLKGKI 1320
ENRFFKLHGS QLSGYHEISR KAKIDINLLK VTKVLRNEDI QADNGGQRNF TDWVLFNECF 1380
QLVFDDGERI TFNAECSNEE KSDWYNKLQE VVELNVFHQP WVKKYCEKLA EEEKTRTTGH 1440
NLKQDFN 1447 
Gene Ontology
 GO:0000142; C:cellular bud neck contractile ring; IDA:SGD.
 GO:0005525; F:GTP binding; IMP:SGD.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0007120; P:axial cellular bud site selection; IMP:SGD.
 GO:0097271; P:protein localization to bud neck; IMP:SGD. 
Interpro
 IPR012966; DUF1709.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 
Pfam
 PF08174; Anillin
 PF00169; PH 
SMART
 SM00233; PH 
PROSITE
 PS50003; PH_DOMAIN 
PRINTS