CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021644
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase Nek6 
Protein Synonyms/Alias
 Never in mitosis A-related kinase 6; NimA-related protein kinase 6; Protein kinase SID6-1512 
Gene Name
 NEK6 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
208RVMHRDIKPANVFITubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. 
Sequence Annotation
 DOMAIN 45 310 Protein kinase.
 NP_BIND 51 59 ATP (By similarity).
 REGION 1 44 Interaction with ARHGAP33, ANKRA2, CDC42,
 REGION 267 270 Interaction with APBB1.
 ACT_SITE 172 172 Proton acceptor (By similarity).
 BINDING 74 74 ATP (By similarity).
 MOD_RES 37 37 Phosphoserine.
 MOD_RES 202 202 Phosphothreonine.
 MOD_RES 206 206 Phosphoserine; by NEK9.
 MOD_RES 210 210 Phosphothreonine.  
Keyword
 Alternative splicing; Apoptosis; ATP-binding; Cell cycle; Cell division; Chromosome partition; Complete proteome; Cytoplasm; Cytoskeleton; Kinase; Magnesium; Metal-binding; Microtubule; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 313 AA 
Protein Sequence
MPRREVCWEA AHFRQEEQSL PRPRVRALVR LACRMAGQPG HMPHGGSSNN LCHTLGPVHP 60
PDPQRHPNTL SFRCSLADFQ IEKKIGRGQF SEVYKATCLL DRKTVALKKV QIFEMMDAKA 120
RQDCVKEIGL LKQLNHPNII KYLDSFIEDN ELNIVLELAD AGDLSQMIKY FKKQKRLIPE 180
RTVWKYFVQL CSAVEHMHSR RVMHRDIKPA NVFITATGVV KLGDLGLGRF FSSETTAAHS 240
LVGTPYYMSP ERIHENGYNF KSDIWSLGCL LYEMAALQSP FYGDKMNLFS LCQKIEQCDY 300
PPLPGEHYSE KLRELVSMCI CPDPHQRPDI GYVHQVAKQM HIWMSST 347 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; IMP:UniProtKB.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IDA:UniProtKB.
 GO:0000910; P:cytokinesis; TAS:UniProtKB.
 GO:0031572; P:G2 DNA damage checkpoint; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0007077; P:mitotic nuclear envelope disassembly; TAS:Reactome.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IDA:UniProtKB.
 GO:2000772; P:regulation of cellular senescence; TAS:UniProtKB.
 GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IDA:UniProtKB.
 GO:0051225; P:spindle assembly; TAS:UniProtKB. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS
 PR00109; TYRKINASE.