CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012841
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycogen synthase kinase-3 alpha 
Protein Synonyms/Alias
 GSK-3 alpha; Serine/threonine-protein kinase GSK3A 
Gene Name
 Gsk3a 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
372TKVFKSSKTPPEAIAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. 
Sequence Annotation
 DOMAIN 119 404 Protein kinase.
 NP_BIND 125 133 ATP (By similarity).
 ACT_SITE 244 244 Proton acceptor (By similarity).
 BINDING 148 148 ATP (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 2 2 Phosphoserine (By similarity).
 MOD_RES 21 21 Phosphoserine; by PKB/AKT1.
 MOD_RES 72 72 Phosphoserine (By similarity).
 MOD_RES 77 77 Phosphoserine (By similarity).
 MOD_RES 97 97 Phosphoserine (By similarity).
 MOD_RES 279 279 Phosphotyrosine (By similarity).  
Keyword
 Acetylation; ATP-binding; Carbohydrate metabolism; Complete proteome; Glycogen metabolism; Kinase; Neurogenesis; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Signal transduction inhibitor; Transferase; Wnt signaling pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 490 AA 
Protein Sequence
MSGGGPSGGG PGGSGRARTS SFAEPGGGGG GGGGGPGGSA SGPGGTGGGK ASVGAMGGGV 60
GASSSGGGPS GSGGGGSGGP GAGTSFPPPG VKLGRDSGKV TTVVATVGQG PERSQEVAYT 120
DIKVIGNGSF GVVYQARLAE TRELVAIKKV LQDKRFKNRE LQIMRKLDHC NIVRLRYFFY 180
SSGEKKDELY LNLVLEYVPE TVYRVARHFT KAKLITPIIY IKVYMYQLFR SLAYIHSQGV 240
CHRDIKPQNL LVDPDTAVLK LCDFGSAKQL VRGEPNVSYI CSRYYRAPEL IFGATDYTSS 300
IDVWSAGCVL AELLLGQPIF PGDSGVDQLV EIIKVLGTPT REQIREMNPN YTEFKFPQIK 360
AHPWTKVFKS SKTPPEAIAL CSSLLEYTPS SRLSPLEACA HSFFDELRRL GAQLPNDRPL 420
PPLFNFSPGE LSIQPSLNAI LIPPHLRSPA GPASPLTTSY NPSSQALTEA QTGQDWQPSD 480
ATTATLASSS 490 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:MGI.
 GO:0050321; F:tau-protein kinase activity; IEA:EC.
 GO:0003214; P:cardiac left ventricle morphogenesis; IMP:BHF-UCL.
 GO:0016477; P:cell migration; IGI:MGI.
 GO:0071285; P:cellular response to lithium ion; IDA:MGI.
 GO:0071407; P:cellular response to organic cyclic compound; IDA:MGI.
 GO:0005977; P:glycogen metabolic process; IEA:UniProtKB-KW.
 GO:0044027; P:hypermethylation of CpG island; IMP:BHF-UCL.
 GO:0008286; P:insulin receptor signaling pathway; IDA:BHF-UCL.
 GO:0061052; P:negative regulation of cell growth involved in cardiac muscle cell development; IMP:BHF-UCL.
 GO:0032007; P:negative regulation of TOR signaling cascade; IMP:BHF-UCL.
 GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
 GO:0071879; P:positive regulation of adrenergic receptor signaling pathway; IMP:BHF-UCL.
 GO:0030819; P:positive regulation of cAMP biosynthetic process; IMP:BHF-UCL.
 GO:2000467; P:positive regulation of glycogen (starch) synthase activity; IMP:BHF-UCL.
 GO:0045823; P:positive regulation of heart contraction; IMP:BHF-UCL.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IDA:MGI.
 GO:0010800; P:positive regulation of peptidyl-threonine phosphorylation; IDA:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IMP:BHF-UCL.
 GO:0006349; P:regulation of gene expression by genetic imprinting; IMP:BHF-UCL.
 GO:0003073; P:regulation of systemic arterial blood pressure; IMP:BHF-UCL.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS