UniProt Accession

 ATG3_HUMAN; Q9NT62; Q6PKC5; Q9H6L9 

Genbank Protein ID

 AB079384; AF202092; AL137515; BC002830; BC024221; AK025778 

Genbank Nucleotide ID

 BAB90843.1; AAG35611.1; CAB70781.1; AAH02830.1; AAH24221.1; BAB15237.1 

Protein Name

 Ubiquitin-like-conjugating enzyme ATG3 

Protein Synonyms/Alias

 Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96 

Gene Name

 ATG3 

Gene Synonyms/Alias

 APG3; APG3L 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	QNVINTVKGKALEVA	ubiquitination	[1]
11	VINTVKGKALEVAEY	ubiquitination	[1]
71	KAYLPTGKQFLVTKN	ubiquitination	[2]
131	KEITLENKDNIRLQD	ubiquitination	[1, 3]
185	IVEACKAKTDAGGED	ubiquitination	[1, 2, 4, 5]
243	ISQDHVKKTVTIENH	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1- E2 complex with ATG3. 

Sequence Annotation

 ACT_SITE 264 264 Glycyl thioester intermediate (Probable).
 MOD_RES 1 1 N-acetylmethionine.
 CROSSLNK 243 243 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Alternative splicing; Autophagy; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Protein transport; Reference proteome; Transport; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 314 AA 

Protein Sequence

Gene Ontology

 GO:0000153; C:cytoplasmic ubiquitin ligase complex; IPI:MGI.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0019777; F:Atg12 ligase activity; ISS:UniProtKB.
 GO:0019776; F:Atg8 ligase activity; ISS:UniProtKB.
 GO:0000045; P:autophagic vacuole assembly; ISS:UniProtKB.
 GO:0043653; P:mitochondrial fragmentation involved in apoptotic process; ISS:UniProtKB.
 GO:0006612; P:protein targeting to membrane; ISS:UniProtKB.
 GO:0016567; P:protein ubiquitination; IDA:UniProtKB. 

Interpro

 IPR007135; Autophagy-rel_prot_3.
 IPR019461; Autophagy-rel_prot_3_C.
 IPR007134; Autophagy-rel_prot_3_N. 

Pfam

 PF03987; Autophagy_act_C
 PF10381; Autophagy_Cterm
 PF03986; Autophagy_N 

SMART

  

PROSITE

  

PRINTS