CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015498
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein 574 
Protein Synonyms/Alias
  
Gene Name
 ZNF574 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
405KTFVNLTKFLYHRRTubiquitination[1, 2, 3]
477LCSREFGKALQLTRHubiquitination[2, 4]
530FPCPDCSKPFNSPANubiquitination[2]
558YRCGDCGKAFTQSSTubiquitination[2, 4, 5]
608HTGEYPYKCRECPRSubiquitination[2, 4]
700GPGEVLAKEPPAPRAubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 ZN_FING 16 38 C2H2-type 1.
 ZN_FING 76 98 C2H2-type 2.
 ZN_FING 126 148 C2H2-type 3.
 ZN_FING 214 236 C2H2-type 4.
 ZN_FING 309 331 C2H2-type 5.
 ZN_FING 336 358 C2H2-type 6.
 ZN_FING 364 386 C2H2-type 7.
 ZN_FING 392 413 C2H2-type 8.
 ZN_FING 466 489 C2H2-type 9.
 ZN_FING 495 517 C2H2-type 10; degenerate.
 ZN_FING 523 545 C2H2-type 11.
 ZN_FING 551 573 C2H2-type 12.
 ZN_FING 579 601 C2H2-type 13.
 ZN_FING 607 630 C2H2-type 14.
 ZN_FING 636 659 C2H2-type 15; degenerate.
 ZN_FING 667 689 C2H2-type 16.
 ZN_FING 738 760 C2H2-type 17.
 ZN_FING 766 788 C2H2-type 18.
 ZN_FING 794 816 C2H2-type 19.
 ZN_FING 822 844 C2H2-type 20.
 MOD_RES 164 164 Phosphoserine.
 MOD_RES 298 298 Phosphoserine.
 MOD_RES 717 717 Phosphoserine.
 MOD_RES 724 724 Phosphothreonine.
 MOD_RES 728 728 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 896 AA 
Protein Sequence
MTEESEETVL YIEHRYVCSE CNQLYGSLEE VLMHQNSHVP QQHFELVGVA DPGVTVATDT 60
ASGTGLYQTL VQESQYQCLE CGQLLMSPSQ LLEHQELHLK MMAPQEAVPA EPSPKAPPLS 120
SSTIHYECVD CKALFASQEL WLNHRQTHLR ATPTKAPAPV VLGSPVVLGP PVGQARVAVE 180
HSYRKAEEGG EGATVPSAAA TTTEVVTEVE LLLYKCSECS QLFQLPADFL EHQATHFPAP 240
VPESQEPALQ QEVQASSPAE VPVSQPDPLP ASDHSYELRN GEAIGRDRRG RRARRNNSGE 300
AGGAATQELF CSACDQLFLS PHQLQQHLRS HREGVFKCPL CSRVFPSPSS LDQHLGDHSS 360
ESHFLCVDCG LAFGTEALLL AHRRAHTPNP LHSCPCGKTF VNLTKFLYHR RTHGVGGVPL 420
PTTPVPPEEP VIGFPEPAPA ETGEPEAPEP PVSEETSAGP AAPGTYRCLL CSREFGKALQ 480
LTRHQRFVHR LERRHKCSIC GKMFKKKSHV RNHLRTHTGE RPFPCPDCSK PFNSPANLAR 540
HRLTHTGERP YRCGDCGKAF TQSSTLRQHR LVHAQHFPYR CQECGVRFHR PYRLLMHRYH 600
HTGEYPYKCR ECPRSFLLRR LLEVHQLVVH AGRQPHRCPS CGAAFPSSLR LREHRCAAAA 660
AQAPRRFECG TCGKKVGSAA RLQAHEAAHA AAGPGEVLAK EPPAPRAPRA TRAPVASPAA 720
LGSTATASPA APARRRGLEC SECKKLFSTE TSLQVHRRIH TGERPYPCPD CGKAFRQSTH 780
LKDHRRLHTG ERPFACEVCG KAFAISMRLA EHRRIHTGER PYSCPDCGKS YRSFSNLWKH 840
RKTHQQQHQA AVRQQLAEAE AAVGLAVMET AVEALPLVEA IEIYPLAEAE GVQISG 896 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS