CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017070
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Poly [ADP-ribose] polymerase 9 
Protein Synonyms/Alias
 PARP-9; ADP-ribosyltransferase diphtheria toxin-like 9; ARTD9; B aggressive lymphoma protein 
Gene Name
 PARP9 
Gene Synonyms/Alias
 BAL; BAL1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
75HNDFKILKNNERQLCubiquitination[1]
105PVQEGNSKSLQVFRKubiquitination[1]
166FEIQEESKQFVARYGubiquitination[1]
174QFVARYGKVSAGEIAubiquitination[1]
191GAGRLPCKQIIHAVGubiquitination[1, 2]
206PRWMEWDKQGCTGKLubiquitination[1]
361QQAGVEMKSEFLATKubiquitination[1]
477PTDLEIYKAFSSEMAubiquitination[1]
485AFSSEMAKRSKMLSLubiquitination[1]
488SEMAKRSKMLSLNNYubiquitination[1]
632QWTIQQQKTQDEMKEubiquitination[1, 3, 4, 5]
638QKTQDEMKENIIFLKubiquitination[1]
659QELLDQKKQFEKCGLubiquitination[1]
663DQKKQFEKCGLQVLKubiquitination[1]
740GAGIYFTKNLKNLAEubiquitination[1, 3, 5]
743IYFTKNLKNLAEKAKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 In concert with DTX3L plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. Involved in inducing the expression of IFN-gamma-responsive genes. 
Sequence Annotation
 DOMAIN 107 296 Macro 1.
 DOMAIN 306 487 Macro 2.
 DOMAIN 628 850 PARP catalytic.  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; DNA damage; DNA repair; NAD; Nucleus; Polymorphism; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 854 AA 
Protein Sequence
MDFSMVAGAA AYNEKSGRIT SLSLLFQKVF AQIFPQWRKG NTEECLPYKC SETGALGENY 60
SWQIPINHND FKILKNNERQ LCEVLQNKFG CISTLVSPVQ EGNSKSLQVF RKMLTPRIEL 120
SVWKDDLTTH AVDAVVNAAN EDLLHGGGLA LALVKAGGFE IQEESKQFVA RYGKVSAGEI 180
AVTGAGRLPC KQIIHAVGPR WMEWDKQGCT GKLQRAIVSI LNYVIYKNTH IKTVAIPALS 240
SGIFQFPLNL CTKTIVETIR VSLQGKPMMS NLKEIHLVSN EDPTVAAFKA ASEFILGKSE 300
LGQETTPSFN AMVVNNLTLQ IVQGHIEWQT ADVIVNSVNP HDITVGPVAK SILQQAGVEM 360
KSEFLATKAK QFQRSQLVLV TKGFNLFCKY IYHVLWHSEF PKPQILKHAM KECLEKCIEQ 420
NITSISFPAL GTGNMEIKKE TAAEILFDEV LTFAKDHVKH QLTVKFVIFP TDLEIYKAFS 480
SEMAKRSKML SLNNYSVPQS TREEKRENGL EARSPAINLM GFNVEEMYEA HAWIQRILSL 540
QNHHIIENNH ILYLGRKEHD ILSQLQKTSS VSITEIISPG RTELEIEGAR ADLIEVVMNI 600
EDMLCKVQEE MARKKERGLW RSLGQWTIQQ QKTQDEMKEN IIFLKCPVPP TQELLDQKKQ 660
FEKCGLQVLK VEKIDNEVLM AAFQRKKKMM EEKLHRQPVS HRLFQQVPYQ FCNVVCRVGF 720
QRMYSTPCDP KYGAGIYFTK NLKNLAEKAK KISAADKLIY VFEAEVLTGF FCQGHPLNIV 780
PPPLSPGAID GHDSVVDNVS SPETFVIFSG MQAIPQYLWT CTQEYVQSQD YSSGPMRPFA 840
QHPWRGFASG SPVD 854 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0003950; F:NAD+ ADP-ribosyltransferase activity; IDA:UniProtKB.
 GO:0016477; P:cell migration; TAS:UniProtKB.
 GO:0006302; P:double-strand break repair; IMP:UniProtKB.
 GO:0060330; P:regulation of response to interferon-gamma; IDA:UniProtKB. 
Interpro
 IPR002589; A1pp.
 IPR012317; Poly(ADP-ribose)pol_cat_dom. 
Pfam
 PF01661; Macro 
SMART
 SM00506; A1pp 
PROSITE
 PS51154; MACRO
 PS51059; PARP_CATALYTIC 
PRINTS