UniProt Accession

 AP2A2_RAT; P18484 

Genbank Protein ID

 X53773 

Genbank Nucleotide ID

 CAA37791.1 

Protein Name

 AP-2 complex subunit alpha-2 

Protein Synonyms/Alias

 100 kDa coated vesicle protein C; Adapter-related protein complex 2 alpha-2 subunit; Adaptor protein complex AP-2 subunit alpha-2; Alpha-adaptin C; Alpha2-adaptin; Clathrin assembly protein complex 2 alpha-C large chain; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit 

Gene Name

 Ap2a2 

Gene Synonyms/Alias

 Adtab 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
855	QEVQNIFKAKHPMDT	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C- terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity). 

Sequence Annotation

 REGION 5 80 Lipid-binding.
 BINDING 43 43 Phosphatidylinositol lipid headgroup (By
 BINDING 53 53 Phosphatidylinositol lipid headgroup (By
 BINDING 57 57 Phosphatidylinositol lipid headgroup (By
 BINDING 58 58 Phosphatidylinositol lipid headgroup (By
 BINDING 61 61 Phosphatidylinositol lipid headgroup (By
 MOD_RES 807 807 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Cell membrane; Coated pit; Complete proteome; Direct protein sequencing; Endocytosis; Lipid-binding; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 938 AA 

Protein Sequence

Gene Ontology

 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0005905; C:coated pit; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0006886; P:intracellular protein transport; IEA:InterPro. 

Interpro

 IPR017104; AP2_complex_asu.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR013038; Clathrin_a-adaptin_app_Ig-like.
 IPR003164; Clathrin_a-adaptin_app_sub_C.
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR015873; Clathrin_a/coatomer_app_sub_C.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like. 

Pfam

 PF01602; Adaptin_N
 PF02296; Alpha_adaptin_C
 PF02883; Alpha_adaptinC2 

SMART

 SM00809; Alpha_adaptinC2 

PROSITE

  

PRINTS