UniProt Accession

 HMP_ECOLI; P24232 

Genbank Protein ID

 X58872; U00096; AP009048; J01620 

Genbank Nucleotide ID

 CAA41682.1; AAC75605.1; BAA16460.1; AAA23911.1 

Protein Name

 Flavohemoprotein 

Protein Synonyms/Alias

 Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD 

Gene Name

 hmp 

Gene Synonyms/Alias

 fsrB; hmpA; b2552; JW2536 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
23	LLVETGPKLTAHFYD	acetylation	[1]
40	FTHNPELKEIFNMSN	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress. 

Sequence Annotation

 DOMAIN 150 255 FAD-binding FR-type.
 NP_BIND 204 207 FAD.
 NP_BIND 268 273 NADP (By similarity).
 NP_BIND 389 392 FAD.
 REGION 1 138 Globin.
 REGION 147 396 Reductase.
 REGION 259 396 NAD or NADP-binding.
 ACT_SITE 95 95 Charge relay system.
 ACT_SITE 135 135 Charge relay system.
 METAL 85 85 Iron (heme proximal ligand).
 BINDING 188 188 FAD.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Detoxification; Direct protein sequencing; FAD; Flavoprotein; Heme; Iron; Metal-binding; NAD; NADP; Oxidoreductase; Oxygen transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 396 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0071949; F:FAD binding; IDA:EcoCyc.
 GO:0005504; F:fatty acid binding; IDA:EcoCyc.
 GO:0020037; F:heme binding; IDA:EcoCyc.
 GO:0032843; F:hydroperoxide reductase activity; IDA:EcoCyc.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0008941; F:nitric oxide dioxygenase activity; IDA:EcoCyc.
 GO:0019825; F:oxygen binding; IEA:InterPro.
 GO:0005344; F:oxygen transporter activity; IEA:HAMAP.
 GO:0051409; P:response to nitrosative stress; IMP:EcoCyc.
 GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW. 

Interpro

 IPR017927; Fd_Rdtase_FAD-bd.
 IPR000971; Globin.
 IPR009050; Globin-like.
 IPR012292; Globin_dom.
 IPR023950; Hmp.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR001433; OxRdtase_FAD/NAD-bd.
 IPR001221; Phe_hydroxylase.
 IPR017938; Riboflavin_synthase-like_b-brl. 

Pfam

 PF00970; FAD_binding_6
 PF00042; Globin
 PF00175; NAD_binding_1 

SMART

  

PROSITE

 PS51384; FAD_FR
 PS01033; GLOBIN 

PRINTS

 PR00410; PHEHYDRXLASE.