UniProt Accession

 EPS15_MOUSE; P42567; Q8C431 

Genbank Protein ID

 L21768; AK083176; AL669905 

Genbank Nucleotide ID

 AAA02912.1; BAC38796.1; CAM13653.1 

Protein Name

 Epidermal growth factor receptor substrate 15 

Protein Synonyms/Alias

 Protein Eps15; Protein AF-1p 

Gene Name

 Eps15 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
227	VSPAEKAKYDEIFLK	ubiquitination	[1]
386	RESINLQKLQAQKQQ	ubiquitination	[1]
449	SYEEELLKAREELSR	ubiquitination	[1]
472	EESVESGKAQLEPLQ	ubiquitination	[1]
638	FKDDPFGKIDPFGGD	ubiquitination	[1]
795	PGKRPINKLDSSDPL	ubiquitination	[1]
803	LDSSDPLKLNDPFQP	ubiquitination	[1]
818	FPGNDSPKEKDPDMF	ubiquitination	[1]
820	GNDSPKEKDPDMFCD	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta- 1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2. 

Sequence Annotation

 DOMAIN 15 104 EH 1.
 DOMAIN 128 216 EH 2.
 DOMAIN 160 195 EF-hand 1.
 DOMAIN 223 258 EF-hand 2.
 DOMAIN 224 314 EH 3.
 REPEAT 599 601 1.
 REPEAT 623 625 2.
 REPEAT 629 631 3.
 REPEAT 634 636 4.
 REPEAT 640 642 5.
 REPEAT 645 647 6.
 REPEAT 651 653 7.
 REPEAT 665 667 8.
 REPEAT 673 675 9.
 REPEAT 693 695 10.
 REPEAT 711 713 11.
 REPEAT 806 808 12.
 REPEAT 827 829 13.
 REPEAT 852 871 UIM 1.
 REPEAT 878 897 UIM 2.
 REGION 2 330 Interaction with DAB2.
 REGION 599 829 13 X 3 AA repeats of D-P-F.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 108 108 Phosphoserine (By similarity).
 MOD_RES 140 140 Phosphoserine (By similarity).
 MOD_RES 323 323 Phosphoserine (By similarity).
 MOD_RES 324 324 Phosphoserine.
 MOD_RES 467 467 Phosphoserine (By similarity).
 MOD_RES 470 470 Phosphoserine (By similarity).
 MOD_RES 485 485 Phosphoserine (By similarity).
 MOD_RES 561 561 Phosphoserine.
 MOD_RES 562 562 Phosphoserine.
 MOD_RES 779 779 Phosphothreonine.
 MOD_RES 781 781 Phosphothreonine.
 MOD_RES 798 798 Phosphoserine (By similarity).
 MOD_RES 816 816 Phosphoserine.
 MOD_RES 850 850 Phosphotyrosine; by EGFR.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Calcium; Cell membrane; Coated pit; Complete proteome; Cytoplasm; Direct protein sequencing; Endocytosis; Endosome; Membrane; Metal-binding; Phosphoprotein; Protein transport; Reference proteome; Repeat; SH3-binding; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 897 AA 

Protein Sequence

Gene Ontology

 GO:0030122; C:AP-2 adaptor complex; IDA:UniProtKB.
 GO:0060170; C:cilium membrane; IDA:MGI.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0048268; P:clathrin coat assembly; IEA:Compara.
 GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR000261; EPS15_homology.
 IPR003903; Ubiquitin-int_motif. 

Pfam

  

SMART

 SM00054; EFh
 SM00027; EH
 SM00726; UIM 

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS50031; EH
 PS50330; UIM 

PRINTS