CPLM-012060

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-012060

UniProt Accession

PABP4_HUMAN; Q13310; B1ANQ8; Q4VC03; Q6P0N3

Genbank Protein ID

U33818; U75686; AL365277; AL845289; AL365277; AL845289; AL845289; AL365277; AL845289; AL365277; CH471059; CH471059; BC065540; BC094755

Genbank Nucleotide ID

AAC50350.1; AAB97309.1; CAI16412.1; CAI16412.1; CAI16414.1; CAI16414.1; CAI12298.1; CAI12298.1; CAI12300.1; CAI12300.1; EAX07263.1; EAX07264.1; AAH65540.1; AAH94755.1

Protein Name

Polyadenylate-binding protein 4

Protein Synonyms/Alias

PABP-4; Poly(A)-binding protein 4; Activated-platelet protein 1; APP-1; Inducible poly(A)-binding protein; iPABP

Gene Name

PABPC4

Gene Synonyms/Alias

APP1; PABP4

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
78	TMNFDVIKGKPIRIM	ubiquitination	[1]
80	NFDVIKGKPIRIMWS	ubiquitination	[1]
95	QRDPSLRKSGVGNVF	ubiquitination	[1]
104	GVGNVFIKNLDKSID	ubiquitination	[1]
108	VFIKNLDKSIDNKAL	ubiquitination	[1]
186	REAELGAKAKEFTNV	ubiquitination	[1]
188	AELGAKAKEFTNVYI	ubiquitination	[1]
216	ELFSQFGKTLSVKVM	ubiquitination	[1]
279	RQAELKRKFEQLKQE	ubiquitination	[1]
284	KRKFEQLKQERISRY	ubiquitination	[1]
309	DDTIDDEKLRKEFSP	ubiquitination	[1]
312	IDDEKLRKEFSPFGS	ubiquitination	[1]
361	NGRIVGSKPLYVALA	ubiquitination	[1]
375	AQRKEERKAHLTNQY	ubiquitination	[1]

Reference

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]

Functional Description

Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo (By similarity).

Sequence Annotation

DOMAIN 11 89 RRM 1.
DOMAIN 99 175 RRM 2.
DOMAIN 191 268 RRM 3.
DOMAIN 294 370 RRM 4.
DOMAIN 551 628 PABC.
MOD_RES 140 140 Phosphotyrosine.
MOD_RES 315 315 Phosphoserine.
MOD_RES 361 361 N6,N6-dimethyllysine.
MOD_RES 518 518 Dimethylated arginine; alternate.
MOD_RES 518 518 Omega-N-methylated arginine; alternate.
MOD_RES 531 531 Phosphoserine.

Keyword

Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Methylation; Phosphoprotein; Polymorphism; Reference proteome; Repeat; RNA-binding.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

644 AA

Protein Sequence

MNAAASSYPM ASLYVGDLHS DVTEAMLYEK FSPAGPVLSI RVCRDMITRR SLGYAYVNFQ 60
QPADAERALD TMNFDVIKGK PIRIMWSQRD PSLRKSGVGN VFIKNLDKSI DNKALYDTFS 120
AFGNILSCKV VCDENGSKGY AFVHFETQEA ADKAIEKMNG MLLNDRKVFV GRFKSRKERE 180
AELGAKAKEF TNVYIKNFGE EVDDESLKEL FSQFGKTLSV KVMRDPNGKS KGFGFVSYEK 240
HEDANKAVEE MNGKEISGKI IFVGRAQKKV ERQAELKRKF EQLKQERISR YQGVNLYIKN 300
LDDTIDDEKL RKEFSPFGSI TSAKVMLEDG RSKGFGFVCF SSPEEATKAV TEMNGRIVGS 360
KPLYVALAQR KEERKAHLTN QYMQRVAGMR ALPANAILNQ FQPAAGGYFV PAVPQAQGRP 420
PYYTPNQLAQ MRPNPRWQQG GRPQGFQGMP SAIRQSGPRP TLRHLAPTGN APASRGLPTT 480
TQRVGSECPD RLAMDFGGAG AAQQGLTDSC QSGGVPTAVQ NLAPRAAVAA AAPRAVAPYK 540
YASSVRSPHP AIQPLQAPQP AVHVQGQEPL TASMLAAAPP QEQKQMLGER LFPLIQTMHS 600
NLAGKITGML LEIDNSELLH MLESPESLRS KVDEAVAVLQ AHHAKKEAAQ KVGAVAAATS 660

Gene Ontology

GO:0010494; C:cytoplasmic stress granule; IDA:MGI.
GO:0005634; C:nucleus; IDA:MGI.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0008143; F:poly(A) RNA binding; IDA:MGI.
GO:0008266; F:poly(U) RNA binding; IDA:MGI.
GO:0007596; P:blood coagulation; TAS:ProtInc.
GO:0006401; P:RNA catabolic process; TAS:ProtInc.
GO:0006396; P:RNA processing; TAS:ProtInc.
GO:0006412; P:translation; TAS:ProtInc.

Interpro

IPR012677; Nucleotide-bd_a/b_plait.
IPR006515; PABP_1234.
IPR002004; PABP_HYD.
IPR000504; RRM_dom.

Pfam

PF00658; PABP
PF00076; RRM_1

SMART

SM00517; PolyA
SM00360; RRM

PROSITE

PS51309; PABC
PS50102; RRM

PRINTS