CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020962
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 5'-3' exoribonuclease 2 
Protein Synonyms/Alias
 Protein Dhm1 
Gene Name
 Xrn2 
Gene Synonyms/Alias
 Dhm1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
286LPREKKGKHDELADSacetylation[1]
493PSPLGGIKRKAEDSDubiquitination[2]
533DVDAADEKFRRKVVQubiquitination[2]
641AIDLNGKKYAWQGVAacetylation[3]
688GDVLFVGKLHPLRDFubiquitination[2]
796SSNGRQWKPQLGFNRubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription (By similarity). 
Sequence Annotation
 ZN_FING 262 278 CCHC-type.
 MOD_RES 439 439 Phosphothreonine (By similarity).
 MOD_RES 448 448 Phosphoserine.
 MOD_RES 471 471 Phosphoserine (By similarity).
 MOD_RES 473 473 Phosphoserine (By similarity).
 MOD_RES 475 475 Phosphoserine (By similarity).
 MOD_RES 499 499 Phosphoserine.
 MOD_RES 501 501 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Exonuclease; Hydrolase; Metal-binding; mRNA processing; Nuclease; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Transcription termination; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 951 AA 
Protein Sequence
MGVPAFFRWL SRKYPSIIVN CVEEKPKECN GVKIPVDASK PNPNDVEFDN LYLDMNGIIH 60
PCTHPEDKPA PKNEDEMMVA IFEYIDRLFN IVRPRRLLYM AIDGVAPRAK MNQQRSRRFR 120
ASKEGMEAAV EKQRVREEIL AKGGFLPPEE IKERFDSNCI TPGTEFMDNL AKCLRYYIAD 180
RLNNDPGWKN LTVILSDASA PGEGEHKIMD YIRRQRAQPN HDPNTHHCLC GADADLIMLG 240
LATHEPNFTI IREEFKPNKP KPCALCNQFG HEVKDCEGLP REKKGKHDEL ADSLPCAEGE 300
FIFLRLNVLR EYLERELTMA SLPFPFDVER SIDDWVFMCF FVGNDFLPHL PSLEIREGAI 360
DRLVNIYKNV VHKTGGYLTE SGYVNLQRVQ MIMLAVGEVE DSIFKKRKDD EDSFRRRQKE 420
KRKRMKRDQP AFTPSGILTP HALGSRNSPG CQVASNPRQA AYEMRMQRNS SPSISPNTSF 480
ASDGSPSPLG GIKRKAEDSD SEPEPEDNVR LWEAGWKQRY YKNKFDVDAA DEKFRRKVVQ 540
SYVEGLCWVL RYYYQGCASW KWYYPFHYAP FASDFEGIAD MSSEFEKGTK PFKPLEQLMG 600
VFPAASGNFL PPTWRKLMSD PDSSIIDFYP EDFAIDLNGK KYAWQGVALL PFVDERRLRA 660
ALEEVYPDLT PEENRRNSLG GDVLFVGKLH PLRDFILELY QTGSTEPVDV PPELCHGIQG 720
TFSLDEEAIL PDQTVCSPVP MLRDLTQNTA VSINFKDPQF AEDYVFKAAM LPGARKPATV 780
LKPGDWEKSS NGRQWKPQLG FNRDRRPVHL DQAAFRTLGH VTPRGSGTSV YTNTALPPAN 840
YQGNNYRPLL RGQAQIPKLM SNMRPQDSWR GPPPLFQQHR FERSVGAEPL LPWNRMIQNQ 900
NAAFQPNQYQ MLGGPGGYPP RRDDHRGGRQ GYPREGRKYP LPPPSGRYSW N 951 
Gene Ontology
 GO:0016235; C:aggresome; IEA:Compara.
 GO:0005730; C:nucleolus; ISS:UniProtKB.
 GO:0004534; F:5'-3' exoribonuclease activity; NAS:UniProtKB.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0016049; P:cell growth; IGI:UniProtKB.
 GO:0000738; P:DNA catabolic process, exonucleolytic; ISS:UniProtKB.
 GO:0006310; P:DNA recombination; NAS:UniProtKB.
 GO:0006281; P:DNA repair; NAS:UniProtKB.
 GO:0006353; P:DNA-dependent transcription, termination; IEA:UniProtKB-KW.
 GO:0007017; P:microtubule-based process; TAS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016070; P:RNA metabolic process; IGI:UniProtKB.
 GO:0007283; P:spermatogenesis; IEP:UniProtKB. 
Interpro
 IPR027073; 5_3_exoribonuclease.
 IPR017151; 5_3_exoribonuclease_2.
 IPR004859; Put_53exo.
 IPR001878; Znf_CCHC. 
Pfam
 PF03159; XRN_N 
SMART
 SM00343; ZnF_C2HC 
PROSITE
 PS50158; ZF_CCHC 
PRINTS