UniProt Accession

 MBD4_HUMAN; O95243; D3DNC3; D3DNC4; Q2MD36; Q7Z4T3; Q96F09 

Genbank Protein ID

 AF072250; AF120999; AF120997; AF120998; AF114784; AM180876; AF532602; CR450305; AF494057; AL449212; CH471052; CH471052; CH471052; CH471052; BC011752 

Genbank Nucleotide ID

 AAC68879.1; AAD50374.1; AAD50374.1; AAD50374.1; AAD22195.1; CAJ55826.1; AAP97338.1; CAG29301.1; AAM00008.1; EAW79251.1; EAW79253.1; EAW79254.1; EAW79255.1; AAH11752.1 

Protein Name

 Methyl-CpG-binding domain protein 4 

Protein Synonyms/Alias

 Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase 

Gene Name

 MBD4 

Gene Synonyms/Alias

 MED1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
56	DEEQMMIKRSSECNP	ubiquitination	[1]
397	RKDFTGEKIFQEDTI	ubiquitination	[1]
484	VLWKFLEKYPSAEVA	ubiquitination	[1, 2, 3]
504	RDVSELLKPLGLYDL	ubiquitination	[1]
526	FSDEYLTKQWKYPIE	ubiquitination	[1]
554	IFCVNEWKQVHPEDH	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein. 

Sequence Annotation

 DOMAIN 76 148 MBD.
 ACT_SITE 560 560 By similarity.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; DNA damage; DNA repair; DNA-binding; Hydrolase; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 580 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004520; F:endodeoxyribonuclease activity; TAS:ProtInc.
 GO:0008263; F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity; IDA:MGI.
 GO:0003696; F:satellite DNA binding; TAS:ProtInc.
 GO:0045008; P:depyrimidination; TAS:Reactome.
 GO:0031572; P:G2 DNA damage checkpoint; IEA:Compara.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IEA:Compara.
 GO:0009314; P:response to radiation; IEA:Compara. 

Interpro

 IPR016177; DNA-bd_integrase-typ.
 IPR011257; DNA_glycosylase.
 IPR003265; HhH-GPD_domain.
 IPR017352; Me_CpG-bd_MBD4.
 IPR001739; Methyl_CpG_DNA-bd. 

Pfam

 PF00730; HhH-GPD
 PF01429; MBD 

SMART

 SM00391; MBD 

PROSITE

 PS50982; MBD 

PRINTS