CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001682
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Fatty acid desaturase 2 
Protein Synonyms/Alias
 Delta(6) fatty acid desaturase; D6D; Delta(6) desaturase; Delta-6 desaturase 
Gene Name
 FADS2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
28FSWEEIQKHNLRTDRubiquitination[1, 2, 3, 4, 5, 6]
42RWLVIDRKVYNITKWubiquitination[4, 5, 6]
48RKVYNITKWSIQHPGubiquitination[2, 3, 4, 5, 6]
84PDLEFVGKFLKPLLIubiquitination[2, 4, 5]
87EFVGKFLKPLLIGELubiquitination[1, 2, 3, 4, 5]
105EPSQDHGKNSKITEDubiquitination[3, 5]
108QDHGKNSKITEDFRAubiquitination[1, 3, 4, 5, 7]
118EDFRALRKTAEDMNLubiquitination[5]
192LSVYRKPKWNHLVHKubiquitination[5]
199KWNHLVHKFVIGHLKubiquitination[5]
206KFVIGHLKGASANWWubiquitination[2, 5]
223RHFQHHAKPNIFHKDubiquitination[1, 4, 5]
229AKPNIFHKDPDVNMLubiquitination[7]
398MPRHNLHKIAPLVKSubiquitination[1, 2, 3, 4, 5, 8]
404HKIAPLVKSLCAKHGubiquitination[2, 3, 4, 5, 8]
409LVKSLCAKHGIEYQEubiquitination[3, 4, 5]
417HGIEYQEKPLLRALLubiquitination[1, 2, 3, 4, 5, 7]
432DIIRSLKKSGKLWLDubiquitination[5]
435RSLKKSGKLWLDAYLubiquitination[2, 3, 4, 5]
444WLDAYLHK*******ubiquitination[4, 7, 8]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3). Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma- linoleic acid (GLA) (18:3n-6) and stearidonic acid (18:4n-3) respectively and other desaturation steps. Highly unsaturated fatty acids (HUFA) play pivotal roles in many biological functions. It catalizes as well the introduction of a cis double bond in palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum. 
Sequence Annotation
 DOMAIN 18 95 Cytochrome b5 heme-binding.
 MOTIF 180 184 Histidine box-1.
 MOTIF 217 221 Histidine box-2.
 MOTIF 382 386 Histidine box-3.  
Keyword
 Alternative splicing; Complete proteome; Electron transport; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 444 AA 
Protein Sequence
MGKGGNQGEG AAEREVSVPT FSWEEIQKHN LRTDRWLVID RKVYNITKWS IQHPGGQRVI 60
GHYAGEDATD AFRAFHPDLE FVGKFLKPLL IGELAPEEPS QDHGKNSKIT EDFRALRKTA 120
EDMNLFKTNH VFFLLLLAHI IALESIAWFT VFYFGNGWIP TLITAFVLAT SQAQAGWLQH 180
DYGHLSVYRK PKWNHLVHKF VIGHLKGASA NWWNHRHFQH HAKPNIFHKD PDVNMLHVFV 240
LGEWQPIEYG KKKLKYLPYN HQHEYFFLIG PPLLIPMYFQ YQIIMTMIVH KNWVDLAWAV 300
SYYIRFFITY IPFYGILGAL LFLNFIRFLE SHWFVWVTQM NHIVMEIDQE AYRDWFSSQL 360
TATCNVEQSF FNDWFSGHLN FQIEHHLFPT MPRHNLHKIA PLVKSLCAKH GIEYQEKPLL 420
RALLDIIRSL KKSGKLWLDA YLHK 444 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0020037; F:heme binding; IEA:InterPro.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0004768; F:stearoyl-CoA 9-desaturase activity; IEA:Compara.
 GO:0036109; P:alpha-linolenic acid metabolic process; TAS:Reactome.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW.
 GO:0043651; P:linoleic acid metabolic process; TAS:Reactome.
 GO:0006636; P:unsaturated fatty acid biosynthetic process; TAS:ProtInc. 
Interpro
 IPR001199; Cyt_B5-like_heme/steroid-bd.
 IPR012171; Fatty_acid/sphinglp_desaturase.
 IPR005804; Fatty_acid_desaturase-1. 
Pfam
 PF00173; Cyt-b5
 PF00487; FA_desaturase 
SMART
  
PROSITE
 PS00191; CYTOCHROME_B5_1
 PS50255; CYTOCHROME_B5_2 
PRINTS