UniProt Accession

 SPF27_HUMAN; O75934; Q6FGS0 

Genbank Protein ID

 AF081788; AB020623; CR542037; BT019390; AL390241; BC005285; BC012623; BC022880 

Genbank Nucleotide ID

 AAC64059.1; BAA34863.1; CAG46834.1; AAV38197.1; CAI13553.1; AAH05285.1; AAH12623.1; AAH22880.1 

Protein Name

 Pre-mRNA-splicing factor SPF27 

Protein Synonyms/Alias

 Breast carcinoma-amplified sequence 2; DNA amplified in mammary carcinoma 1 protein; Spliceosome-associated protein SPF 27 

Gene Name

 BCAS2 

Gene Synonyms/Alias

 DAM1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	TRRYRPTKNYLSYLT	ubiquitination	[1, 2]
85	PIELLSMKRYELPAP	ubiquitination	[1]
97	PAPSSGQKNDITAWQ	ubiquitination	[2, 3]
158	KELQKLRKHIQDLNW	ubiquitination	[3, 4]
177	MQLTAGSKLREMESN	ubiquitination	[1]
191	NWVSLVSKNYEIERT	ubiquitination	[1]
210	ENEIYQIKQQHGEAN	ubiquitination	[1]
218	QQHGEANKENIRQDF	ubiquitination	[3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Polymorphism; Reference proteome; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 225 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IDA:MGI.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0000375; P:RNA splicing, via transesterification reactions; TAS:UniProtKB. 

Interpro

 IPR008409; SPF27. 

Pfam

 PF05700; BCAS2 

SMART

  

PROSITE

  

PRINTS