CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001518
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein HEXIM1 
Protein Synonyms/Alias
 Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Hexamethylene bis-acetamide-inducible protein 1; Menage a quatre protein 1 
Gene Name
 HEXIM1 
Gene Synonyms/Alias
 CLP1; EDG1; HIS1; MAQ1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
165SKKKRHWKPYYKLTWacetylation[1, 2]
196IRAEMFAKGQPVAPYubiquitination[3]
284ESLQNMSKQELIKEYacetylation[4]
296KEYLELEKCLSRMEDacetylation[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. 
Sequence Annotation
 REGION 150 177 Basic region; mediates nuclear
 REGION 202 205 Interaction with P-TEFb.
 REGION 210 250 Autoinhibitory acidic region; in absence
 REGION 286 314 Mediates interaction with CCNT1.
 REGION 310 355 Required for inhibition of ESR1-dependent
 MOD_RES 97 97 Phosphoserine.
 MOD_RES 98 98 Phosphoserine.
 MOD_RES 233 233 Phosphoserine.
 MOD_RES 236 236 Phosphothreonine.
 MOD_RES 237 237 Phosphoserine.
 MOD_RES 252 252 Phosphoserine.  
Keyword
 3D-structure; Coiled coil; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 359 AA 
Protein Sequence
MAEPFLSEYQ HQPQTSNCTG AAAVQEELNP ERPPGAEERV PEEDSRWQSR AFPQLGGRPG 60
PEGEGSLESQ PPPLQTQACP ESSCLREGEK GQNGDDSSAG GDFPPPAEVE PTPEAELLAQ 120
PCHDSEASKL GAPAAGGEEE WGQQQRQLGK KKHRRRPSKK KRHWKPYYKL TWEEKKKFDE 180
KQSLRASRIR AEMFAKGQPV APYNTTQFLM DDHDQEEPDL KTGLYSKRAA AKSDDTSDDD 240
FMEEGGEEDG GSDGMGGDGS EFLQRDFSET YERYHTESLQ NMSKQELIKE YLELEKCLSR 300
MEDENNRLRL ESKRLGGDDA RVRELELELD RLRAENLQLL TENELHRQQE RAPLSKFGD 359 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0005634; C:nucleus; IDA:HGNC.
 GO:0004861; F:cyclin-dependent protein serine/threonine kinase inhibitor activity; IDA:HGNC.
 GO:0017069; F:snRNA binding; IDA:HGNC.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0045736; P:negative regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:HGNC.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:HGNC.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR024872; HEXIM. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02094; HEXIMFAMILY.