UniProt Accession

 KIN10_ARATH; Q38997; A6XGR0; O04728; Q38987; Q39076; Q8RWD2 

Genbank Protein ID

 M93023; X94757; DQ778957; AC008261; CP002686; CP002686; CP002686; AY093170; BT010386; X79707; X86966 

Genbank Nucleotide ID

 AAA32736.1; CAA64384.1; ABH11527.1; AAF26165.1; AEE73607.1; AEE73608.1; AEE73609.1; AAM13169.1; AAQ56829.1; CAA56146.1; CAA60529.1 

Protein Name

 SNF1-related protein kinase catalytic subunit alpha KIN10 

Protein Synonyms/Alias

 AKIN10; AKIN alpha-2; AKINalpha2 

Gene Name

 KIN10 

Gene Synonyms/Alias

 AK21; SKIN10; SNR2; SNRK1.1; At3g01090; T4P13.22 

Created Date

 July 27, 2013 

Organism

 Arabidopsis thaliana (Mouse-ear cress) 

NCBI Taxa ID

 3702 

Lysine Modification

Position	Peptide	Type	References
43	ESILPNYKLGRTLGI	ubiquitination	[1]

Reference

 [1] Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants.
 Maor R, Jones A, Nühse TS, Studholme DJ, Peck SC, Shirasu K.
 Mol Cell Proteomics. 2007 Apr;6(4):601-10. [PMID: 17272265] 

Functional Description

 Catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN10 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination. Phosphorylates GRIK1/SNAK2 and GRIK2/SNAK1 in vitro. 

Sequence Annotation

 DOMAIN 42 294 Protein kinase.
 DOMAIN 315 355 UBA.
 DOMAIN 486 534 KA1.
 NP_BIND 48 56 ATP (By similarity).
 ACT_SITE 165 165 Proton acceptor (By similarity).
 BINDING 71 71 ATP (By similarity).
 MOD_RES 198 198 Phosphothreonine; by GRIK1 or GRIK2.
 CROSSLNK 43 43 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Alternative splicing; ATP-binding; Carbohydrate metabolism; Complete proteome; Fatty acid biosynthesis; Fatty acid metabolism; Isopeptide bond; Kinase; Lipid biosynthesis; Lipid metabolism; Nitrate assimilation; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 535 AA 

Protein Sequence

Gene Ontology

 GO:0000152; C:nuclear ubiquitin ligase complex; IPI:TAIR.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:TAIR.
 GO:0009738; P:abscisic acid mediated signaling pathway; IMP:TAIR.
 GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
 GO:0009594; P:detection of nutrient; IDA:TAIR.
 GO:0003006; P:developmental process involved in reproduction; IMP:TAIR.
 GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
 GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
 GO:0010260; P:organ senescence; IMP:TAIR.
 GO:0080022; P:primary root development; IMP:TAIR.
 GO:0010182; P:sugar mediated signaling pathway; IMP:TAIR.
 GO:0010050; P:vegetative phase change; IMP:TAIR. 

Interpro

 IPR001772; KA1_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 

Pfam

 PF02149; KA1
 PF00069; Pkinase
 PF00627; UBA 

SMART

 SM00220; S_TKc
 SM00165; UBA 

PROSITE

 PS50032; KA1
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50030; UBA 

PRINTS