CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007297
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ammonium transporter MEP1 
Protein Synonyms/Alias
  
Gene Name
 MEP1 
Gene Synonyms/Alias
 AMT1; YGR121C; G6331 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
480GEMIQSEKILPIHQEubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
 Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. Can also transport methylamine. The affinity of MEP1 is about twenty times lower than that of MEP2. MEP3 has the lowest affinity. 
Sequence Annotation
 MOD_RES 442 442 Phosphoserine.
 MOD_RES 445 445 Phosphoserine.  
Keyword
 Ammonia transport; Complete proteome; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 492 AA 
Protein Sequence
MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL ALIWVVLMAT 60
LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK KFDEDAYPEL AYATFQMMFS 120
CVNLSIIAGA TAERGRLLPH MVFLFILATI GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI 180
EILSAVSGFV YSWFLGKRNE KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS 240
VYAFMNTCLS AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ 300
GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI GMDGTTEHEG 360
GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG MRLRISEEAE EAGMDEDQIG 420
EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR VNNAHLAAER SSSGTNSSSD GNGEMIQSEK 480
ILPIHQEDPA NR 492 
Gene Ontology
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; ISS:SGD.
 GO:0008519; F:ammonium transmembrane transporter activity; IMP:SGD.
 GO:0019740; P:nitrogen utilization; IMP:SGD. 
Interpro
 IPR001905; Ammonium_transpt.
 IPR018047; Ammonium_transpt_CS.
 IPR024041; NH4_transpt_AmtB-like_dom. 
Pfam
 PF00909; Ammonium_transp 
SMART
  
PROSITE
 PS01219; AMMONIUM_TRANSP 
PRINTS