UniProt Accession

 MED6_HUMAN; O75586; O15401; Q53FE3; Q53HJ3; Q6FHQ4; Q9BTH1; Q9UHL1 

Genbank Protein ID

 AF074723; BT006831; CR541697; AK222587; AK223346; BC004106; U78082; AF155104 

Genbank Nucleotide ID

 AAC26869.1; AAP35477.1; CAG46498.1; BAD96307.1; BAD97066.1; AAH04106.1; AAB84363.1; AAD42870.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 6 

Protein Synonyms/Alias

 Activator-recruited cofactor 33 kDa component; ARC33; Mediator complex subunit 6; hMed6; Renal carcinoma antigen NY-REN-28 

Gene Name

 MED6 

Gene Synonyms/Alias

 ARC33 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
200	VQLKPGEKPVPVDQT	ubiquitination	[1]
236	VQQTVSAKGPPEKRM	acetylation	[2]
241	SAKGPPEKRMRLQ**	acetylation	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

 MOD_RES 236 236 N6-acetyllysine.
 MOD_RES 241 241 N6-acetyllysine.  

Keyword

 Acetylation; Activator; Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 246 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IMP:UniProtKB.
 GO:0003677; F:DNA binding; IEA:Compara.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro.
 GO:0003713; F:transcription coactivator activity; IDA:MGI.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:MGI.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR007018; Mediator_Med6.
 IPR016820; Mediator_Med6_met/pln. 

Pfam

 PF04934; Med6 

SMART

  

PROSITE

  

PRINTS