CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007178
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 5B 
Protein Synonyms/Alias
 eIF-5B; Translation initiation factor IF-2 
Gene Name
 FUN12 
Gene Synonyms/Alias
 YAL035W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
298ENASEAIKSDSKKDSubiquitination[1]
457DAIKAKTKVMAEYEKubiquitination[1]
714IAANDLEKAVSGSRLacetylation[2]
714IAANDLEKAVSGSRLubiquitination[1]
794VYKRDVMKASTMLEKubiquitination[3]
817CFDVKVDKEAEQYAEacetylation[2]
817CFDVKVDKEAEQYAEubiquitination[1]
907VKTDPTTKERQTLILubiquitination[1]
976RRSIDTLKDKAFRDQacetylation[2]
993RSDWLLLKKLKVVFGacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2. 
Sequence Annotation
 NP_BIND 412 419 GTP (By similarity).
 MOD_RES 405 405 Phosphoserine.  
Keyword
 Complete proteome; GTP-binding; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1002 AA 
Protein Sequence
MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA SAEGAEAIEG 60
DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE KEKQKKKEQA ARKKAQQQAQ 120
KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG ESDKPSASAK KPAKKVPAGL AALRRQLELK 180
KQLEEQEKLE REEEERLEKE EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK 240
QKEEKKLLER RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 300
SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN EDQNQGEEEE 360
EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK KDLRSPICCI LGHVDTGKTK 420
LLDKIRQTNV QGGEAGGITQ QIGATYFPID AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH 480
ESFSNLRSRG SSLCNIAILV IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA 540
IPNNSFRDSF AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 600
TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI LSNGYLREGD 660
RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK AALGVKIAAN DLEKAVSGSR 720
LLVVGPEDDE DELMDDVMDD LTGLLDSVDT TGKGVVVQAS TLGSLEALLD FLKDMKIPVM 780
SIGLGPVYKR DVMKASTMLE KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF 840
DSFTAYQEKL LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 900
KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ PIWGRHVDEN 960
DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG IE 1002 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005525; F:GTP binding; IDA:SGD.
 GO:0003924; F:GTPase activity; IMP:SGD.
 GO:0043022; F:ribosome binding; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IDA:SGD.
 GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD.
 GO:0006446; P:regulation of translational initiation; IMP:SGD. 
Interpro
 IPR000795; EF_GTP-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR015760; TIF_IF2.
 IPR023115; TIF_IF2_dom3.
 IPR004161; Transl_elong_EFTu/EF1A_2.
 IPR009000; Transl_elong_init/rib_B-barrel. 
Pfam
 PF00009; GTP_EFTU
 PF03144; GTP_EFTU_D2
 PF11987; IF-2 
SMART
  
PROSITE
 PS01176; IF2 
PRINTS
 PR00315; ELONGATNFCT.