CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001238
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TIP41-like protein 
Protein Synonyms/Alias
 Putative MAPK-activating protein PM10; Type 2A-interacting protein; TIP 
Gene Name
 TIPRL 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19DFCFGPWKLTASKTHacetylation[1]
19DFCFGPWKLTASKTHubiquitination[2]
24PWKLTASKTHIMKSAubiquitination[2]
29ASKTHIMKSADVEKLubiquitination[2]
84NNYQGMLKVACAEEWubiquitination[1, 2]
102RTEGEHSKEVIKPYDubiquitination[2]
106EHSKEVIKPYDWTYTacetylation[3]
106EHSKEVIKPYDWTYTubiquitination[1, 2, 4, 5, 6, 7]
117WTYTTDYKGTLLGESubiquitination[1, 2, 4, 5, 7, 8, 9, 10]
126TLLGESLKLKVVPTTubiquitination[2, 5, 8, 10]
128LGESLKLKVVPTTDHubiquitination[1, 2, 5, 9, 10]
140TDHIDTEKLKAREQIubiquitination[1, 2]
142HIDTEKLKAREQIKFubiquitination[2]
207RLYHEADKTYMLREYacetylation[3]
207RLYHEADKTYMLREYubiquitination[1, 2, 4, 7]
251IKEAVCEKLIFPERIacetylation[1]
251IKEAVCEKLIFPERIubiquitination[2]
267PNPADSQKSTQVE**ubiquitination[1, 2, 5, 8, 9]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 May be a allosteric regulator of serine/threonine- protein phosphatase 2A (PP2A). Isoform 1 inhibits catalytic activity of the PP2A(D) core complex in vitro. The PP2A(C):TIPRL complex does not show phosphatase activity. May play a role in the regulation of ATM/ATR signaling pathway controlling DNA replication and repair. 
Sequence Annotation
 REGION 173 272 Interaction with PPP2CA.
 MOD_RES 106 106 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 272 AA 
Protein Sequence
MMIHGFQSSH RDFCFGPWKL TASKTHIMKS ADVEKLADEL HMPSLPEMMF GDNVLRIQHG 60
SGFGIEFNAT DALRCVNNYQ GMLKVACAEE WQESRTEGEH SKEVIKPYDW TYTTDYKGTL 120
LGESLKLKVV PTTDHIDTEK LKAREQIKFF EEVLLFEDEL HDHGVSSLSV KIRVMPSSFF 180
LLLRFFLRID GVLIRMNDTR LYHEADKTYM LREYTSRESK ISSLMHVPPS LFTEPNEISQ 240
YLPIKEAVCE KLIFPERIDP NPADSQKSTQ VE 272 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0000077; P:DNA damage checkpoint; IMP:UniProtKB.
 GO:0034048; P:negative regulation of protein phosphatase type 2A activity; IDA:UniProtKB. 
Interpro
 IPR007303; TIP41-like. 
Pfam
 PF04176; TIP41 
SMART
  
PROSITE
  
PRINTS